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MABF2690-100UL Anti-CD40 Antibody, clone FGK45.5

MABF2690-100UL
100 µL  
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      Overview

      Description
      Catalogue NumberMABF2690-100UL
      DescriptionAnti-CD40 Antibody, clone FGK45.5
      Alternate Names
      • Tumor necrosis factor receptor superfamily member 5
      • B-cell surface antigen CD40
      • Bp50
      • CD40L receptor
      Background InformationTumor necrosis factor receptor superfamily member 5 (UniProt: P27512; also known as B-cell surface antigen CD40, Bp50, CD40L receptor, CD40) is encoded by the Cd40 (also known as Tnfrsf5) gene (Gene ID: 21939) in murine species. CD40 is a single-pass type I transmembrane glycoprotein that serves as a receptor for TNFSF5/CD40L. It is a costimulatory protein found on antigen-presenting cells and is required for their activation. Its expression has been observed in B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. It transduces TRAF-6 and MAP3K8-mediated signals that activate ERK in macrophages and B cells that leads to induction of immunoglobulin secretion. CD40 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-193), a transmembrane domain (aa 194-205), and a cytoplasmic domain (aa 216-289). Five isoforms of CD40 have been described that are produced by alternative splicing.
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationThis rat monoclonal Anti-CD40, clone FGK45.5, Cat. No. MABF2690, is a rat monoclonal antibody that detects Tumor necrosis factor receptor superfamily member 5 (CD40) and is tested for use in Flow Cytometry and Function Analysis.
      Key Applications
      • Flow Cytometry
      • Affects Function
      Application NotesFlow Cytometry Analysis: A representative lot detected CD40 in Flow Cytometry applications (Rolink, A., et. al. (1996). Immunity. 5(4):319-30).

      Function Analysis: A representative lot promoted antigen presenting cell (APC) maturation and enhanced macrophage tumoricidal activity (Luheshi, N.M., et. al. (2016). Oncotarget. 7(14):18508-20).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant mouse CD40 fusion protein.
      EpitopeUnknown
      CloneFGK45.5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone FGK45.5 is a rat monoclonal antibody that detects Tumor necrosis factor receptor superfamily member 5 (CD40).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cd40
      • Tnfrsf5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight32.09 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

      Flow Cytometry Analysis: 1 µg of this antibody detected CD40 in one million C57BL/6 mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2690-100UL 04061842378180