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AM03T Anti-Bak (Ab-1) Mouse mAb (TC-100)

Anti-Bak (Ab-1) Mouse mAb (TC-100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: bcl-2 Antagonist Killer

AM03T
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~30 (apparent MW) kDa Bak protein in HL-60 cells.
      Catalogue NumberAM03T
      Brand Family Calbiochem®
      Synonymsbcl-2 Antagonist Killer
      References
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol, pH 7.5.
      Positive controlHL-60 cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (2.5 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems for each application.
      Biological Information
      Immunogenrecombinant human Bak protein from which the C-terminal and transmembrane domains have been deleted
      ImmunogenHuman
      Epitopewithin the N-terminal region
      CloneTC-100
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM03T 0

      Documentation

      Anti-Bak (Ab-1) Mouse mAb (TC-100) Certificates of Analysis

      TitleLot Number
      AM03T

      References

      Reference overview
      Chittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      Synonymsbcl-2 Antagonist Killer
      ApplicationImmunoblotting (2.5 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 (apparent MW) kDa Bak protein.
      BackgroundBak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. Bak was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa but migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA3. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner, has been found in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human Bak protein from which the C-terminal and transmembrane domains have been deleted
      Epitopewithin the N-terminal region
      CloneTC-100
      IsotypeIgG2a
      Specieshuman, not mouse, not rat
      Positive controlHL-60 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems for each application.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies