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MABS2046-25UG Anti-ApoB Antibody, clone 2G11

Detects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting.

Anti-ApoB Antibody, clone 2G11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Apob UniProt Number: E9Q414
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MABS2046-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HtELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2046-25UG
      DescriptionAnti-ApoB Antibody, clone 2G11
      Alternate Names
      • Apolipoprotein B-100
      Background InformationApolipoprotein B-100 (UniProt: E9Q414; also known as Apo B-100) is encoded by the Apob gene (Gene ID: 238055) in murine species. Apolipoprotein B is a major protein constituent of chylomicrons. Apo B particles are made up of lipids and proteins and each Apo B is bordered by a phospholipid monolayer with an inner core composed of variable amounts of triglycerides and cholesterol esters. Apo B is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off. Two isoforms of Apo B are produced- Apo B-100 is synthesized in the liver and Apo B-48 is produced in the intestine. Apo B-100 contains the region that binds to the LDL receptor. It contains VLDL particles, VLDL remnant particles and LDL particles. On the other hand, the Apo B-48 particles are made of chylomicrons and chylomicron remnant particles. Generally, LDL particles account for approximately 90% of Apo B particles in plasma. Abnormal trapping of an Apo B is a major factor in the development of atherosclerotic lesions. When Apo B particles degrade cholesterol is retained with macrophages that induce inflammatory response. Further entry and entrapment of Apo B can promote cardiovascular pathology over time. (Ref.: Sniderman, A et al. (2010). Nat. Rev. Endocrinol. 6(6); 335-346).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern Blotting Analysis: 4 µg/mL from a representative lot detected ApoB in 10 µg of rat liver and mouse liver tissue lysate.
      Western Blotting Analysis: A representative lot detected ApoB in Western Blotting applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5; Cheng, D., et. al. (2016). J Biol Chem. 291(45):23793-23803).
      ELISA Analysis: A representative lot detected ApoB in ELISA applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5).
      Biological Information
      ImmunogenApoB-containing lipoproteins isolated from Apoe -/- Apob 48/48 mice.
      Clone2G11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2G11 detects Apolipoprotein B in Mouse, Rat, and Hamster plasma and tissues. It binds to ApoB-48 and ApoB-100, but not to ApoB-39.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Hamster
      Species Reactivity NoteMouse, Rat, Hamster. Does not react with human.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Apob
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~260 kDa observed; 509.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat serum.
      Western Blotting Analysis: 4 µg/mL of this antibody detected ApoB in 10 µg of rat serum.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2046-25UG 04054839551178