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ABD43 Anti-Apelin receptor Antibody

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ABD43
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, PIA, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABD43
      DescriptionAnti-Apelin receptor Antibody
      Alternate Names
      • Apelin Receptor
      • Angiotensin Receptor-Like 1
      • G-Protein Coupled Receptor Apj
      • G-Protein Coupled Receptor Hg11
      Background InformationApelin receptor (UniProt: P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID: 187) in human. Apelin receptor(AR), a monomeric transmembrane protein, is a class A GPCR that is widely distributed in human. It is also expressed in embryonic and induced pluripotent stem cells. It is activated by its cognate peptide ligand, apelin, and induces a wide variety of effects, including vasoconstriction and dilation, strengthening cardiac muscle contractility, angiogenesis, fluid homeostasis, and energy metabolism. It also plays role in heart morphogenesis and promotes angioblast migration towards embryonic midline. Apelin-AR signaling has cardioprotective effects and administration of apelin-13 to glucose-deprived cultured cardiomyocytes has inhibitory effects on apoptosis. Glutamic acid 20 and aspartic acid 23 at the extracellular N-terminus of AR are reported to be the first physiological sites that bind apelin. After exposure to apelin, the receptor is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. (Ref.: Hu, G., et al. (2021). Front. Physiol. 12; 632886; Wang, Z., et al. (2015). Sci. Rep. 5; 8170; Chapman, NA., et al. (2014). Biochem. Cell Biol. 92(6); 431-440).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse Cerebellum Tissue Lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Apelin receptor, Cat. No. ABD43, is a rabbit polyclonal antibody that detects Apelin receptor and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in Mouse cerebellum, Human cerebral cortex, and Mouse cerebral cortex tissue sections.

      Peptide Inhibition Assay: Target band detection in Mouse cerebellum tissue lysate was prevented by pre-blocking of a representative lot with the immunogen peptide.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the third cytoplasmic domain within the C-terminal half of human Apelin receptor.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Apelin receptor. It targets an epitope within the third cytoplasmic domain from the C-terminal half.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rhesus Macaque, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009].
      Gene Symbol
      • Aplnr
      • Agtrl1
      • Apj
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

      TISSUE SPECIFICITY: Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.

      SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family.
      Molecular Weight~56 kDa observed; 42.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse cerebellum tissue lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apelin receptor in Mouse cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABD43 04053252438974

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-Apelin receptor Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Apelin receptor Antibody Certificates of Analysis

      TitleLot Number
      Anti-Apelin receptor 2475904
      Anti-Apelin receptor - 2150754 2150754
      Anti-Apelin receptor - 2227165 2227165
      Anti-Apelin receptor - 2282192 2282192
      Anti-Apelin receptor - 2329825 2329825
      Anti-Apelin receptor - 2512047 2512047
      Anti-Apelin receptor - 3194623 3194623
      Anti-Apelin receptor - 3308238 3308238
      Anti-Apelin receptor - 3885502 3885502
      Anti-Apelin receptor - 4188735 4188735

      References

      Reference overviewPub Med ID
      Pharmacological and immunohistochemical characterization of the APJ receptor and its endogenous ligand apelin.
      Medhurst, Andrew D, et al.
      J. Neurochem., 84: 1162-72 (2003)  2003

      Show Abstract
      12603839 12603839

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies