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ABE552 Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody

This Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: FTO UniProt Number: Q9C0B1
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ABE552
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE552
      DescriptionAnti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody
      Alternate Names
      • Alpha-ketoglutarate-dependent dioxygenase FTO
      • Fat mass and obesity-associated protein
      Background InformationAlpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated Alpha-ketoglutarate-dependent dioxygenase FTO from 500 μg of U2OS nuclear extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Alpha-ketoglutarate-dependent dioxygenase FTO.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Chimpanzee, Monkey, Ox, and Sheep based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FTO
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~58 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS nuclear extract.

      Western Blotting Analysis: 1 μg/mL of this antibody detected Alpha-ketoglutarate-dependent dioxygenase FTO in 10 μg of U2OS nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE552 04053252945687

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies