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MABE1121 Anti-Ago1 Antibody, clone 4B8

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MABE1121
100 μg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1121
      DescriptionAnti-Ago1 Antibody, clone 4B8
      Alternate Names
      • Protein argonaute-1
      • Argonaute1
      • hAgo1
      • Argonaute RISC catalytic component 1
      • Eukaryotic translation initiation factor 2C 1
      • eIF-2C 1
      • eIF2C 1
      • Putative RNA-binding protein Q99
      • Ago1
      Background InformationProtein argonaute-1 (UniProt Q9UL18; also known as Argonaute1, Argonaute RISC catalytic component 1, eIF-2C 1, eIF2C 1, Eukaryotic translation initiation factor 2C 1, hAgo1, Putative RNA-binding protein Q99) is encoded by the AGO1 (also known as EIF2C, EIF2C1) gene (Gene ID 26523) in human. Ago1 belongs to the family of argonaute proteins involved in gene expression by regulating gene transcription and RNA splicing in the nucleus, as well as by regulating posttranscriptional gene silencing mediated by siRNAs and microRNAs in the cytosol. Ago1 is shown to interact with chromatin modifiers, splicing factors, and RNA polymerase II. Ago1 and the related Ago2 are also shown to target Hepatitis C Virus (HCV) RNÁ 5′-untranslated region (5′-UTR) in an miR-122-dependent manner, resulting in viral RNA stability and translation stimulation.
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Ago1 in 10 µg of K562 cell lysate.
      Western Blotting Analysis: A representative lot detected Ago1-containing messenager ribonucleoprotein (mRNP) complexes primarily in the low density fractions of sucrose gradient-fractionated HEK293 lysate (Höck, J., et al. (2007). EMBO Rep. 8(11):1052-1060).
      Immunoprecipitation Analysis: Representative lots immunoprecipitated FLAG-tagged Ago1, but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).
      RNA Binding Protein Immunoprecipitation (RIP): Representative lots co-immunoprecipitated Ago1-associated RNAs, including mRNAs, miRNAs, and snoRNAs from human cell lysates (Li, Z., et al. (2009). J. Virol. 83(24):12751-12758; Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).
      Biological Information
      ImmunogenGST-tagged recombinant human Ago1.
      Clone4B8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 4B8 sepcifically immunoprecipitated FLAG-tagged Ago1,but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AGO1
      • EIF2C
      • EIF2C1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~97 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ago1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1121 04055977301175