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MAB19310 Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28

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MAB19310
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, RELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB19310
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28
      Background InformationAggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
      Product Information
      FormatPurified
      Control
      • Cartilage, neural tube, and brain tissue
      PresentationLiquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot

      ELISA

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenFFGVGGEEDC-KLH peptide
      EpitopeN-terminus FFGVG neoepitopes
      CloneAF-28
      HostMouse
      SpecificityMouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
      Gene Symbol
      • ACAN
      • MSK16
      • SEDK
      • CSPCP
      • AGCAN
      • AGC1
      • CSPG1
      • CSPGCP
      Non-Reactive Species
      • Horse
      • Guinea Pig
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
      SIZE: 2415 amino acids; 250193 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Restricted to cartilages.
      DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
      DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
      DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
      SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB19310 04053252467509