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ECM445 β1 Integrin Partners Kit

Research Category: Cell Structure Research Sub-Category: Integrins Gene Symbol: ITGB1, GPIIA, VLAB, OTTHUMP00000063732, MDF2, FNRB, OTTHUMP00000063731, OTTHUMP00000046253, CD29, OTTHUMP00000063733, MSK12 UniProt Number: P05556
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ECM445
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      Description
      Catalogue NumberECM445
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Descriptionβ1 Integrin Partners Kit
      OverviewChemicon Investigator Kits for Integrin Research are a cost-effective source of reagents for all investigators intimately involved with this expanding field of study. The beta1 Integrin Partners Investigator Kit is designed for research focused on the human beta1 integrin subunit and those integrin subunits known to pair with beta1, including alpha1, alpha2, alpha3, alpha4, alpha5, alpha6 and alphaV.

      Integrins belong to a large family of cell surface proteins commonly referred to as glycoproteins. Integrins are composed of noncovalently associated alpha and beta subunits which make up heterodimers: the larger termed alpha chain and the smaller beta chain. At least 16 alpha subunits and 8 beta subunits are expressed, which can give rise to 22 different integrins. Integrins are thought to play critical roles in cell migration, differentiation, and survival. There is now considerable evidence that these transmembrane receptors play an important role in the regulation of gene expression and signal transduction. Excellent reviews of integrin structure, function, and ligand specificity may be found in: Hynes, R. 1992. Cell 69:11-25; Kramer, R. 1993. "Integrin Structure and Ligand Specificity in Cell-Matrix Interactions" in Molecular and Cellular Aspects of Basement Membranes. Academic Press.

      Chemicon offers the b1 Integrin Partners Investigator Kit for initial studies of b1 subunit interactions. All kit reagents are also available in larger sizes. The reagents in this kit are not application specific but rather may be used in a variety of procedures, depending upon the needs of the individual investigator.

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      References
      Product Information
      Components
      • beta1 Mouse Monoclonal Antibody (Part No. MAB1951Z-20)- 1 tube, 20 μg.
      • alpha1 Mouse Monoclonal Antibody (Part No. MAB1973Z-20) - 1 tube, 20 μg.
      • alpha2 Mouse Monoclonal Antibody (Part No. MAB1950Z-20)- 1 tube, 20 μg.
      • alpha3 Mouse Monoclonal Antibody (Part No. MAB1952Z-20) - 1 tube, 20 μg.
      • alpha4 Mouse Monoclonal Antibody (Part No. MAB16983Z-20) - 1 tube, 20 μg.
      • alpha5 Mouse Monoclonal Antibody (Part No. MAB 1956Z-20) - 1 tube, 20 μg.
      • alpha6 Rat Monoclonal Antibody (Part No. MAB1378-20)- 1 tube, 20 μg.
      • alphaV Mouse Monoclonal Antibody (Part No. MAB1953Z-20)- 1 tube, 20 μg.
      HS Code3002 15 90
      PresentationWith 20 μg each of monoclonals to alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alphaV, and beta1. Chemicon's beta1 Integrin Kit (ECM445) provides a cost-effective means to study the beta1 integrin and its associated alpha subunits.
      Quality LevelMQ100
      Applications
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      • Immunoprecipitation
      • Flow Cytometry
      • Affects Function
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • VLAB
      • OTTHUMP00000063732
      • MDF2
      • FNRB
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • CD29
      • OTTHUMP00000063733
      • MSK12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


      SIZE: 798 amino acids; 88465 Da

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

      TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





      SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore antibodies at -20°C.

      Important Note: Due to the small volumes contained within the kit, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge before opening to dislodge any liquid entrapped in the container's cap during shipment.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ECM445 04053252474569

      Documentation