For over forty years, Estapor® Microspheres has been a leading brand of polymeric supports for in vitro diagnostics, life sciences and biotechnology applications worldwide. Our Estapor® beads offer uniformity, reproducibility, low background & non-specific binding, format flexibility, supply chain reliability and scalability with extensive, application specific technical support.
You can order our most versatile polystyrene based Estapor® microspheres online. Each product/lot conforms to quality level (MQ) 300, and comes in a polyethylene bottle storable at 2-8°C.
Suitable for your applications in:
Lateral Flow (LF) – Europium and Colored (dyed) Microspheres
Chemiluminescent Immunoassay (CLIA) - BioEstapor Microspheres
Immunoturbidimetry Assay (LTIA) - White Microspheres
For the more extensive range of Estapor® products please refer to the brochure(s) and contact your Merck Account Manager or customer service.
Lateral Flow (LF) Europium Microspheres
Estapor is proud to launch our new Europium microspheres. Labeled with Europium, these microspheres provide several advantages over traditional fluorescent microspheres.
- Significantly improve lateral flow assay sensitivity
- Drastically reduce background fluorescence
- Make assays easier to read and quantify
- Exhibit a much longer Stokes shift than traditional fluorescent labels
- Exhibit an enhanced fluorescent quantum yield facilitating a low detection limit
- Are functionalized with a carboxylated surface for protein conjugation
- Are available in three different size options
*** Products only available in certain countries ***
| Description | Diameter | Functional Group | Qty/Pk | Catalog |
|---|---|---|---|---|
| Europium Functionalized Microspheres - F1-EU 010-0,1G-P | 0.130 µm- 0.190 µm | COOH >300 μEq/g | Vialing size: 0,1g | 80380623-0010-P |
| Europium Functionalized Microspheres - F1-EU 030-0,1G-P | 0.270 µm- 0.330 µm | COOH 200 μEq/g – 500 μEq/g | Vialing size: 0,1g | 80380624-0010-P |
| Europium Functionalized Microspheres - F1-EU 050-0,1G-P | 0.450 µm- 0.530 µm | COOH 1 μEq/g - 10 μEq/g | Vialing size: 0,1g |
80380625-0010-P |
Dyed Functionalized Microspheres
Estapor's colored microspheres are best suited for assays where the primary goal is simply detecting the presence or absence of the target analyte. Choose the desired combination of surface functionalization, color and size to find the best microsphere for your application.
| Description | Diameter | Functional Group | Qty/Pk | Catalog |
|---|---|---|---|---|
| Dyed Functionalized Microspheres - K1-030 BLACK F-1G | 0.270 µm–0.330 µm | COOH 200 µEq–500 µEq/g | Vialing size: 1g | 80380143-0010 |
| Dyed Functionalized Microspheres - K1-030 BLUE W-1G | 0.270 µm–0.330 µm | COOH 200 µEq–500 µEq/g | Vialing size: 1g | 80380065-0010 |
| Dyed Functionalized Microspheres - K1-030 RED C1-1G | 0.270 µm–0.330 µm | COOH 200 µEq–500 µEq/g | Vialing size: 1g | 80380011-0010 |
Chemiluminescent Immunoassay (CLIA) Microspheres
Bio-Estapor® microspheres are pre-conjugated to streptavidin facilitating convenient separation, purification and detection of biotinylated biomolecules including antigens, antibodies and nucleic acids from different sources, such as blood, sera, tissues and food.
Alongside streptavidin-conjugation, our Bio-Estapor® microspheres also combine efficient magnetophoretic mobility, separating rapidly in a complex liquid as well as high surface area and low sedimentation rate. The Ferrite Content is 36-50%.
| Description | Catalog | Diameter | Functional Group | Qty/Pk |
|---|---|---|---|---|
| Bio-Estapor Microspheres - BE-M08/0,86-0,01G | F80380096-0001 | 0.900 µm–1.800 µm | "COOH 10 µEq–100 µEq/g" | Vialing size: 0,01g |
Immunoturbidimetry (IT) Microspheres
| Description | Catalog | Diameter | Functional Group | Qty/Pk | |
|---|---|---|---|---|---|
| "Estapor's standard polystyrene Estapor® microspheres offer a simple and convenient option for the development of a wide range Latex Turbidimetry Immunoassay (LTIA) diagnostic tests. The plain surface of the microsphere is designed to be easily and quickly coated with the antibody or antigen of choice via protein adsorption through hydrophobic and ionic interactions. The simplicity of this conjugation process is a key feature of these microspheres and has attracted many diagnostic test developers whom have successfully utilized these microspheres for several years in a wide variety of LTIAs. | |||||
| White Microspheres - K 030-1G | 23690087-0010 | 0.276 µm–0.325 µm | Plain | Vialing size: 1g | |
| White Microspheres - K 007-1G | 39431081-0010 | 0.050 µm - 0.080µm | Plain | Vialing size: 1g | |
| White Microspheres - K 010-1G | 39469081-0010 | 0.081 µm–0.125 µm | Plain | Vialing size: 1g | |
| White Microspheres - K 020-1G | 39430087-0010 | 0.176 µm–0.225 µm | Plain | Vialing size: 1g | |
| Estapor's surfactant-free polystyrene microspheres are an excellent choice for Latex Turbidimetry Immunoassay (LTIA) diagnostic manufacturers for with more stringent requirements. Available in three microsphere size options, our surfactant-free range is highly uniform, exhibits high antibody/antigen binding and offers straightforward handling without the use of surfactant. | |||||
| Surfactant-Free White Microspheres - KSF010-1G | 80380104-0010 | 0.101 µm–0.140 µm | Plain (surfactant free) | Vialing size: 1g | |
| This smaller Estapor microsphere, with its slower agglutination kinetics, is designed to help mitigate against the ‘hook’ or ‘prozone’ effect whereby the effectiveness of antibodies to form immune complexes can be diminished when concentrations of an antibody or an antigen are high. This provides diagnostic developers a further possibility to optimize their assays. Given the high refractive index of the polystyrene microspheres, the particles may exhibit a high degree of scattered light within some assays. | |||||
| White Functionalized Microspheres - PSI-90/21-1G | 80380012-0010 | 0.080 µm – 0.150 µm | COOH 100 µEq–200 µEq/g | Vialing size: 1g | |
*** Products only available in certain countries ***