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70089 pBACgus-3 DNA

70089
  
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      Overview

      Replacement Information
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      Overview

      This product has been discontinued.





      pBACgus-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence iscapable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBACgus-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
      Catalogue Number70089
      Brand Family Novagen®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70089 0

      Documentation

      pBACgus-3 DNA SDS

      Title

      Safety Data Sheet (SDS) 

      pBACgus-3 DNA Certificates of Analysis

      TitleLot Number
      70089

      Brochure

      Title
      Insect Cell Expression

      Citations

      Title
    • Austin N. Kirschner, et al. (2006) Soluble Epstein-Barr virus glycoproteins gH, gL, and gp42 form a 1:1:1 stable complex that acts like soluble gp42 in B-cell fusion but not in epithelial cell fusion. Journal of Virology 80, 9444-9454.
    • Maisoa P. McShane, et al. (2003) Mutational anaylsis of the HLA Class II interaction with Epstein-Barr virus glycoprotein 42. Journal of Virology 77, 7655-7662.
    • Vector Map

      Title
      TB218 pBACgus-3 Vector Map

      Vector Sequence

      Title
      pBACgus-3 Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits