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GF154 Wnt-3a, recombinant mouse

The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.

Wnt-3a, recombinant mouse MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P27467
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GF154
5 µg  
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      Overview

      Key Spec Table

      Key ApplicationsSpeciesSourcePurity
      CULTMouse Mouse L cells expressing full length Wnt3a. Endotoxin level is less than 0.1ng/µg (1EU/µg).
      Description
      Catalogue NumberGF154
      ReplacesGF160
      DescriptionWnt-3a, recombinant mouse
      OverviewRecombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues. When analyzed by SDS-PAGE under non-reducing conditions, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa.

      Wnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. Wnt proteins have also been shown to have putative roles in the regulation of adult stem cells.
      Product Information
      PresentationLyophilized from a solution containing PBS and 1.0% CHAPS buffer.
      Quality LevelMQ100
      Applications
      ApplicationThe recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.
      Key Applications
      • Cell Culture
      Biological Information
      PurityEndotoxin level is less than 0.1ng/µg (1EU/µg).
      SourceMouse L cells expressing full length Wnt3a.
      Specific ActivityWnt3a protein has been shown to be bioactive as determined by TCF/LEF-luciferase reporter. The ED50 for this effect is typically <250 ng/mL. Optimal concentrations should be determined for each application.
      Gene Symbol
      • WNT3A
      UniProt Number
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight38.0 - 41.0 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the lyophilized material at -20°C up to six months from date of receipt. Resuspend in sterile water to a concentration of 100µg/mL. Reconstituted Wnt3a should be stored in single use aliquots at -80°C for up to three months. Avoid repeated freeze/thaw cycles. During shipment, some amount of product will occasionally dispersed. It’s recommended to gently tape the vial on the hard surface before reconstitution.
      Packaging Information
      Material Size5 µg
      Global Trade Item Number
      Catalogue Number GTIN
      GF154 04055977112344