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SCT153 Renilla Luciferase Assay

Flash-type Renilla Luciferase assay designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.

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SCT153
1 kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT153
      DescriptionRenilla Luciferase Assay
      OverviewRenilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues.

      The Renilla Luciferase Assay utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of about 2 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels.
      Alternate Names
      • Firefly Luciferase Assay
      • Bioluminescent Assay
      References
      Product Information
      Components
      • 1) 5X Passive Lysis Buffer (CS224582): 30 mL
      • 2) Renilla Luciferase Assay Buffer 2.0 (CS224583): 100 mL
      • 3) Aquaphile™ Coelenterazine (CS224584): 1 X 4 mg
      Detection methodChemiluminescence
      HS Code3822 19 90
      Presentationliquid
      Applications
      ApplicationFlash-type Renilla Luciferase assay designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.
      Key Applications
      • Cell Based Assays
      Application NotesFirefly Luciferase Assay
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Renilla Luciferase Assay at –80°C. Renilla Luciferase Assay Buffer 2.0 is stable at -80°C for at least six months from date of receipt. Other kit components are stable at -20°C for at least six months from date of receipt. Kit components and stock solutions of Aquaphile coelenterazine in water are stable to at least 5 freeze/thaw cycles.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT153 04054839566059