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528283 Polo-like Kinase Inhibitor II, BTO-1 - CAS 40647-02-7 - Calbiochem

The Polo-like Kinase Inhibitor II, BTO-1, also referenced under CAS 40647-02-7, controls the biological activity of Polo-like Kinase. This small molecule/inhibitor is primarily used for Phosphorylatio

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Synonyms: 5-Cyano-7-nitro-2-(benzothiazolo-N-oxide)-carboxamide

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528283
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Overview

Key Spec Table

CAS #Empirical Formula
40647-02-7C₉H₄N₄O₄S

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      Description
      OverviewA cell-permeable benzothiazolo-N-oxide compound that targets the ATP-binding pocket of polo-like kinase and is shown to inhibit Plk1 kinase activity in cell-free kinase assays (IC50 = 8.0 µM) and suppress the phosphorylation of cellular Plk1 substrate Cdc25C in rat kangaroo kidney-derived PTK cells (75% inhibition at 6.3 µM). BTO-1 treatment of cultured cells results in mitosis defects consistent with loss-of-Plk1 phenotypes seen in Plk1 RNAi-treated U20S cells, including the appearance of monopolar spindles and the reduction of γ-tubulin at the centrosomes. Plk1 inhibition by BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring.
      Catalogue Number528283
      Brand Family Calbiochem®
      Synonyms5-Cyano-7-nitro-2-(benzothiazolo-N-oxide)-carboxamide
      References
      ReferencesBrennan, I.M., et al. 2007. PloS ONE 2, e409.
      Peters, U., et al. 2006. Nat. Chem. Biol. 2, 618.
      McInnes, C., et al. 2006. Nat. Chem. Biol. 2, 608.
      Product Information
      CAS number40647-02-7
      FormBeige to brown solid
      Hill FormulaC₉H₄N₄O₄S
      Chemical formulaC₉H₄N₄O₄S
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Biological Information
      Purity≥97% by HPLC
      Safety Information
      R PhraseR: 20/21/22-36/37/38

      Harmful by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36/37

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and gloves.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Global Trade Item Number
      Catalogue Number GTIN
      528283-2MG 04055977196580