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539732 PTP, T-Cell, Human, Recombinant, E. coli

539732
  
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      Overview

      Replacement Information
      Description
      OverviewRecombinant, human T-cell PTP, truncated at the C-terminus (an 11Kd deletion) and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.
      Catalogue Number539732
      Brand Family Calbiochem®
      SynonymsProtein Tyrosine Phosphatase, T-Cell
      References
      ReferencesGordon, J.A. 1991. Methods Enzymol. 201, 477.
      Zander, N.F., et al. 1991. Biochemistry 30, 6964.
      Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
      Product Information
      Activity≥10,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol of <i>p</i>NPP per min at 30°C, pH 7.0.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% BRIJ® 35 Detergent, 50% glycerol, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥15,000 units/mg
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      539732 0

      Documentation

      PTP, T-Cell, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      PTP, T-Cell, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      539732

      References

      Reference overview
      Gordon, J.A. 1991. Methods Enzymol. 201, 477.
      Zander, N.F., et al. 1991. Biochemistry 30, 6964.
      Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-September-2008 RFH
      SynonymsProtein Tyrosine Phosphatase, T-Cell
      DescriptionRecombinant, human T-cell PTP, truncated at the C-terminus (an 11Kd deletion) and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% BRIJ® 35 Detergent, 50% glycerol, pH 7.0.
      Purity≥95% by SDS-PAGE
      Specific activity≥15,000 units/mg
      Activity≥10,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.0.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGordon, J.A. 1991. Methods Enzymol. 201, 477.
      Zander, N.F., et al. 1991. Biochemistry 30, 6964.
      Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.