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LSKGEL050 Montage Gel Extraction Kit

DNA Gel Extraction

Montage Gel Extraction Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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LSKGEL050
50  
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      Overview

      Description
      Catalogue NumberLSKGEL050
      Trade Name
      • Montage
      DescriptionMontage Gel Extraction Kit
      OverviewRecover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

      The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

      Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

      • Minimal hands-on time
      • 10-minute spin
      • Fully-functional DNA


      Performance
      Typical DNA Recoveries from Agarose Gels

      DNA Size (bp) Percent DNA Recoveries

      100 78
      700 71
      1000 77
      2027 47
      4361 35
      9416 32
      23130 29

      Protocol
      1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
      2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
      3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
      4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
      Alternate Names
      • Ultrafree-DA
      Product Information
      Components
      • includes 50 Ultrafree-DA devices and 500 mL of 50x concentrated modified TAE Buffer
      Color CodeBlue
      Quality LevelMQ300
      Applications
      ApplicationDNA Gel Extraction
      Materials Information
      Device Material
      • Polypropylene
      Packaging Information
      Material Size50
      Global Trade Item Number
      Catalogue Number GTIN
      LSKGEL050 04053252326288