Microcon® Centrifugal Filters
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Overview
Specifications
Ordering Information
Documentation
Related Products & Applications
Related Products By: Application Facete
| Protein Purification |
| DNA Purification |
| Dialysis and Filtration |
Related Products By: Brand Facete
| Ultracel® |
Categories
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
Microcon® Centrifugal Filters
Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:
- Typical recoveries of >95%, even for dilute solutions
- Reverse spin to maximize recovery, even in the smallest samples
- Convenient storage of filtrate or concentrated sample in standard microfuge tube
- Concentration factors up to 100X
Microcon® DNA Fast Flow Filter
Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.
Features:
- High recovery for small volumes with reverse spin (concentration factor <20X)
- Low-binding Ultracel® membrane
- Fast processing
Sample applications:
- Recovery of genomic DNA for forensic applications
- Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
- Removal of primers from amplified DNA
- Easy filtrate recovery, exploiting the flat membrane design
| Microcon® Device | |||
| Application | 10K | 30K | DNA Fast Flow |
| Peptide and growth factor concentration | ♦ | ||
| Protein concentration and desalting of columns eluates | ♦ | ♦ | |
| Protein concentration before electrophoresis or other assays | ♦ | ♦ | |
| Protein removal prior to HPLC | ♦ | ♦ | |
| Purification of macromolecular components found in tissue culture extracts and cell lysates | ♦ | ♦ | |
| Concentration of biological samples (antigens, antibodies, enzymes) | ♦ | ||
| Concentration of gDNA with or without SDS buffer | ♦ | ♦ | |
| Concentration and desalting of nucleic acids (single-or double-stranded) | ♦ | ♦ | ♦ |
| Removal of labeled nucleotides | ♦ | ♦ | ♦ |
| Removal of labeled amino acids | ♦ | ♦ | ♦ |
| Removal of primers from amplified DNA | ♦ | ♦ | |
| Removal of linkers prior to cloning | ♦ | ♦ | |
Applications
Recovery of Genomic DNA for Forensic Applications, Concentration and Desalting of Nucleic Acids (high recovery alternative to ethanol precipitation), Removal of Primers from Amplified DNA, Protein Concentration
| Volumes | |
| Maximum initial sample volume, µL | 0.5 ml |
| Typical final concentration volume, µL | 5 -50 μL |
| OPTIMAL and maximum relative centrifugal force – Fixed angle rotor | Microcon® 10K devices - 14,000 x g Microcon® 30K devices - 14,000 x g Microcon® DNA Fast Flow - 500 x g 1,000 x g for recovery spin |
| Active membrane area, cm2 | 0.32 cm2 |
| Hold up volume, µL | ≤10 μL |
| Materials | |
| Filter device | Polycarbonate |
| Membrane | Ultracel® low-binding regenerated cellulose |
| Membrane support base | Acetal |
| Collection tube | Polypropylene |
| O-Ring | Medical-grade silicone rubber |
| Dimensions | |
| Diameter, mm (in.) | 12.3 mm, (0.5 in.) |
| Length, mm (in.) (filter device and tube in concentration mode) | 45.0 mm (1.8 in.) |
| Length, mm (in.) (filter device and tube in recovery mode) | 48.2 mm (1.9 in.) |
| PES | RC |
| The open microstructure provides extremely fast separation capability for serum, plasma, or conditioned tissue culture media. | The hydrophilic, tight microstructure assures the highest possible retention with the lowest possible adsorption of protein, DNA or other macromolecules |
| For use when concentrating and desalting more concentrated samples or whenever you need to concentrate faster (High dirt loading capacity) | For use when concentrating or desalting extremely dilute solutions or whenever your sample is hydrophobic. |
| Higher liquid flow than RC. | RC is highly sensitive to pH (working range of 4~6.5). Very low protein binding capacity, which makes it an excellent choice for protein recovery applications |
| Low protein and drug binding | Low non-specific adsorption |
| PES has broad solvent compatibility. | RC has broad solvent compatibility |
| Good mechanical strength, thermal stability | Good mechanical strength, thermal stability |
| Hydrophilic | Hydrophilic |
| Low carbohydrate binding | Interacts with many carbohydrate binding proteins as regenerated cellulose has glucose monomers as building block |
| Usually the downstream steps in a purification train will employ PES membrane chemistry for concentration/buffer exchange due to the lack of membranous impurities (PES is known to bind proteins, especially hydrophobic ones) | Upstream Ultrafiltration/diafiltration unit operations will utilize cellulose-based membranes because of less fouling by hydrophobic solutes. |
| Suitable for filtration of aqueous and compatible organic solvents. | Suitable for filtration of aqueous and compatible organic solvents |