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444248 MMP-13, Proenzyme, His•Tag®, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Collagenase-3

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444248
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444248-10UG
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      		 Plastic ampoule 10 μg
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      Description
      OverviewFull-length recombinant, human MMP-13 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca2+- and Zn2+-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).
      Note: 1 mU = 1 milliunit.
      Catalogue Number444248
      Brand Family Calbiochem®
      SynonymsCollagenase-3
      References
      ReferencesHerget, J., et al. 2003. Am. J. Physiol. 285, 4199.
      Nagase, H. 1997. Biol. Chem. 378, 151.
      Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.
      Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
      Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
      Knight, C.G., et al. 1992. FEBS Lett. 296, 263.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (<a href = "/Products/ProductDisplay.asp→catNO=03-32-5032">Cat. No. 03-32-5032</a>) per min at 37°C, pH 7.5.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥50 mU/mg protein
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      444248-10UG 04055977186482