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429700 Leptin, Human, Recombinant, E. coli

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429700
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429700-1MG
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      Description
      OverviewRecombinant, human leptin expressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations of the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Note: Following complete dissolution in 15 mM HCl, add 7.5 mM sterile NaOH and bring the pH to approximately 5.2.
      Catalogue Number429700
      Brand Family Calbiochem®
      SynonymsrhOB
      References
      ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
      Ookuma, M., et al. 1998. Diabetes 47, 219.
      Campfield, L.A., et al. 1995. Science 269, 546.
      Halaas, J.L., et al. 1995. Science 269, 543.
      Pelleymounter, M.A., et al. 1995. Science 269, 540.
      Zhang, Y., et al. 1994. Nature 372, 425.
      Product Information
      CAS number177404-21-6
      FormLyophilized
      FormulationLyophilized from a sterile filtered solution in PBS.
      Quality LevelMQ100
      Biological Information
      Biological activityED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg leptin
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsTo reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
      Global Trade Item Number
      Catalogue Number GTIN
      429700-1MG 04055977210132