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WB64 HeLa Cell Nuclear Extract

WB64
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Nuclear extract derived from HeLa cells, a human epithelioid cervical carcinoma cell line. Useful as a positive control for immunoblots of human nuclear proteins such as transcription factors. Sufficient for 10 gel lanes.
      Catalogue NumberWB64
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 350 mM NaCl, 20 mM HEPES, 5 mM DTT, 1 mM MgCl₂, 0.1 mM EGTA, 20% glycerol, 1% Igepal, protease inhibitors, pH 7.5.
      PreservativeNone
      Applications
      Application NotesImmunoblotting
      Electrophoretic Mobility Shift Assay
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      WB64 0

      Documentation

      HeLa Cell Nuclear Extract Certificates of Analysis

      TitleLot Number
      WB64
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-August-2007 RFH
      ApplicationImmunoblotting
      Electrophoretic Mobility Shift Assay
      DescriptionNuclear extract derived from HeLa cells.
      BackgroundHeLa is a human epithelioid cervical carcinoma cell line. It is useful as a positive control for immunoblots of human nuclear proteins such as transcription factors.
      FormLiquid
      FormulationIn 350 mM NaCl, 20 mM HEPES, 5 mM DTT, 1 mM MgCl₂, 0.1 mM EGTA, 20% glycerol, 1% Igepal, protease inhibitors, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling