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SCC292 HEK293 FUS Knockout Human Cell Line

The HEK293 FUS knockout cell line was derived via CRISPR/Cas9-editing of HEK293 cells. Experimental validation of this line confirmed deletion of FUS with no off-target effects on expression of related proteins.2 This FUS knockout cell line permits direct study of the effects of FUS mutations via transfection of FUS variants. The HEK293 FUS knockout cell line represents a valuable system for the investigation of familial ALS in a robust cellular model.

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SCC292
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC292
      DescriptionHEK293 FUS Knockout Human Cell Line
      OverviewAmyotrophic lateral sclerosis (ALS, Charcot or Lou Gehrig s disease) is a progressive neurodegenerative disease resulting in loss of motor control. ALS usually leads to mortality within 5 years of symptom onset.1 A subset of ALS cases are familial and lin
      Background InformationAmyotrophic lateral sclerosis (ALS, also known as Charcot or Lou Gehrig’s disease) is a progressive neurodegenerative disease resulting in loss of motor control. ALS usually leads to mortality within 5 years of symptom onset.1 A subset of ALS cases are familial and linked to mutations in a handful of genes, including FUS (fused in sarcoma). FUS is a ribonucleotide binding protein that plays a role in RNA metabolism and is implicated in DNA repair pathways.2 Probing the effects of FUS point mutations is contributing to our understanding of the cellular mechanisms of ALS and the connections between genetic repair and neurodegeneration.

      Source
      HEK293 FUS KO cell line was derived from CRISPR-edited HEK293 cells.2 The parental HEK293 cell line was derived from cells from an aborted female fetus transfected with sheared adenovirus DNA.3

      References
      1. Zarei S, Carr K, Reiley L, Diaz K, Guerra O et al. Surg Neurol Int 2015; 6:171.
      2. Wang H, Guo W, Mitra J, Hegde PM, Vandoorne T et al. Nat Commun 2018; 9:3683.
      3. Graham FL, Smiley J, Russell, WC, Nairn R. J Gen Virol 1977; 36(1): 59-74.
      References
      Product Information
      Applications
      ApplicationThe HEK293 FUS knockout cell line was derived via CRISPR/Cas9-editing of HEK293 cells. Experimental validation of this line confirmed deletion of FUS with no off-target effects on expression of related proteins.2 This FUS knockout cell line permits direct study of the effects of FUS mutations via transfection of FUS variants. The HEK293 FUS knockout cell line represents a valuable system for the investigation of familial ALS in a robust cellular model.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsCells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC292 04065266515688