Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Please refer to vial label for lot-specific concentration
Physicochemical Information
Contaminants
None detected by IEP using anti-fibronectin and anti-rat serum
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-May-2008 RFH
Description
Native fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Form
Liquid
Formulation
In 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Procedure for Coating of Tissue Culture Plates with Fibronectin
This procedure is based on the use of 21 cm2 dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and the 1 mg package size of fibronectin. One mg of fibronectin is sufficient to coat 10 dishes at 5 µg/cm2.
1. After thawing the fibronectin solution as indicated above, bring the solution to a final volume of 20 ml with sterile serum-free medium that has been preheated to 37°C. This yields a fibronectin solution of 50 µg/ml.
2. Add 2 ml of this solution to each of 10 tissue culture plates and swirl gently to completely coat the entire growth surface.
3. Allow dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces.
4. Tilt each dish and remove the fibronectin solution using a sterile pipette. Do not permit the pipette tip to disturb the growth surface.
5. Add cell suspension in medium directly to the fibronectin-coated dishes.
This procedure is meant to serve as a guideline and optimal may require optimization.
CAS number
86088-83-7
Purity
single major band by SDS-PAGE
Contaminants
None detected by IEP using anti-fibronectin and anti-rat serum
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
