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322326 Diphtheria Toxin, Unnicked, Corynebacterium diphtheriae - Calbiochem

Diphtheria toxin catalyzes ADP-ribosylation of eukaryotic aminoacyltransferase II (EF2) using NAD as substrate. Activation requires nicking with a protease followed by reduction with DTT.

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322326
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      Description
      OverviewCatalyzes ADP-ribosylation of eukaryotic aminoacyltransferase II (EF2) using NAD as substrate, thereby inhibiting protein synthesis. May also induce internucleosomal breakdown. Causes DNA fragmentation and cytolysis in U937 cells. Activation requires nicking with a protease followed by reduction with DTT.
      Catalogue Number322326
      Brand Family Calbiochem®
      References
      ReferencesKochi, S.K., and Collier, R.J. 1993. Exp. Cell Res. 208, 296.
      Chang, M.P., et al. 1989. J. Biol. Chem. 264, 15261.
      Pappenheimer, A.M., Jr. 1977. Annu. Rev. Biochem. 46, 69.
      Ittelson, T.R., and Gill, D.M. 1973. Nature 242, 330.
      Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
      Pappenheimer, A.M., et al. 1972. Immunochem. 9, 891.
      Uchida, T., et al. 1972. Science 175, 901.
      Bowman, C.G., and Bonventre, P.F. 1970. J. Exp. Med. 131, 659.
      Baseman, J.B., et al. 1970. J. Exp. Med. 132, 1138.
      Gill, D.M., et al. 1969. J. Exp. Med. 129, 1.
      Gill, D.M., et al. 1969. Cold Spring Harbor Symp. Quant. Biol. 34, 589.
      Honjo, J., et al. 1968. J. Biol. Chem. 243, 3553.
      Product Information
      ATP CompetitiveN
      FormSolid
      FormulationLyophilized from sterile 10 mM Tris, 1 mM EDTA, pH 7.5.
      ReversibleN
      Quality LevelMQ100
      Biological Information
      Biological activityNote: Toxicity may vary by lot of toxin. Each laboratory should determine the optimum dosage for each particular application.
      Primary TargetEukaryotic aminoacyltransferase II (EF2)
      PurityMajor band (under reducing conditions) of ~63 kDa
      Physicochemical Information
      Cell permeableN
      Safety Information according to GHS
      RTECSXW5807200
      Safety Information
      R PhraseR: 20/21/22

      Harmful by inhalation, in contact with skin and if swallowed.
      S PhraseS: 36

      Wear suitable protective clothing.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot, quickly freeze on dry ice, and freeze (-70°C). Subsequent thawing should be carried out only at room temperature. For assays employing very low concentrations of diphtheria toxin, the use of a carrier protein, such as BSA or HSA, is recommended.
      Global Trade Item Number
      Catalogue Number GTIN
      322326-1MG 07790788060923