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ABN2271-100UL Anti-poly-(QAGR)

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ABN2271-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2271-100UL
      DescriptionAnti-poly-(QAGR)
      Alternate Names
      • (Gln-Ala-Gly-Arg)Exp
      Background InformationRAN, a small GTP binding protein, belonging to the RAS superfamily is involved in the translocation of RNA and proteins through the nuclear pore complex and participate in the control of DNA synthesis and cell cycle progression. Antisense QAGR (Gln-Ala-Gly-Arg ) RAN proteins that are localized primarily in the nucleus of oligodendrocytes are reported to accumulate within the white matter region of the brain. They are detectable in frontal cortex, basal ganglia, and hippocampus in subjects with myotonic dystrophy type 2 (DM2) but are not detected in normal control brain tissue. QAGR proteins are known to be toxic to cells independent of RNA effects. QAGR RAN proteins also accumulate in regions of brain that display certain neuropathological changes. These include regions with accumulation of activated microglia, increased astrocytes, and white matter rarefaction. Studies have shown that the tetranucleotide DM2 CAGG expansion mutation is bidirectionally transcribed and the resulting RNAs are RAN translated and produce tetrapeptide expansion proteins with QAGR repeats from the antisense strand, which accumulate in brains from DM2 subjects. (Ref.: Zu, T., et al. (2017). Neuron 95 (8); 1292-1305).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide
      Applications
      ApplicationAnti-poly-(QAGR), Cat. No. ABN2271, is a highly specific rabbit polyclonal antibody that targets poly-(QAGR) and has been tested for use in Immunohistochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected poly-(QAGR) in Western Blotting applications (

      Immunofluorescence Analysis: A representative lot detected poly-(QAGR) in Immunofluorescence applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

      Immunohistochemistry Analysis: A representative lot detected poly-(QAGR) in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
      Biological Information
      ImmunogenA synthetic peptide corresponding to the (QAGR)EXP repeat motif.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects proteins with poly-(QAGR).
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Purification MethodUnpurified
      Molecular Weight~26 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293Tcells transfected with poly-(QAGR).


      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected poly-(QAGR) in HEK293T cells transfected with poly-(QAGR), but not in control cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2271-100UL 04054839986703

      Documentation

      Anti-poly-(QAGR) Certificates of Analysis

      TitleLot Number
      Anti-poly-(QAGR) - 3723433 3723433
      Anti-poly-(QAGR) - Q3222900 Q3222900

      References

      Reference overviewPub Med ID
      RAN Translation in Huntington Disease.
      Bañez-Coronel, M; Ayhan, F; Tarabochia, AD; Zu, T; Perez, BA; Tusi, SK; Pletnikova, O; Borchelt, DR; Ross, CA; Margolis, RL; Yachnis, AT; Troncoso, JC; Ranum, LP
      Neuron  88  667-77  2015

      Show Abstract
      26590344 26590344