04-811
Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal
culture supernatant, Upstate®
Synonym(s):
NOS III, endothelial nitric oxidase synthase, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
monoclonal
Species reactivity:
bovine
Application:
WB
Citations:
3
biological source
rabbit
Quality Segment
antibody form
culture supernatant
antibody product type
primary antibodies
clone
monoclonal
species reactivity
bovine
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr495)
Gene Information
mouse ... Nos3(287024)
General description
135 kDa
Immunogen
Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
Application
This Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Western Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
Biochem/physiol Actions
Predicted cross-reactivity with human, rabbit, mouse and rat
Recognizes phosphorylated eNOS/NOS III.
Physical form
Cultured supernatant in 0.05% sodium azide
Analysis Note
Control
Fetal bovine heart endothelial cell lysate
Fetal bovine heart endothelial cell lysate
routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
Other Notes
Replaces: 05-811
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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