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ABS1008 Anti-phospho Glucocorticoid Receptor (Ser134) Antibody

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.

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Research Category: Signaling Research Sub-Category: Hormones & Receptors Gene Symbol: NR3C1, GCCR, GCR, GCRST, GRL UniProt Number: P04150
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ABS1008
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1008
      DescriptionAnti-phospho Glucocorticoid Receptor (Ser134) Antibody
      Alternate Names
      • Glucocorticoid receptor, Ser134-phosphorylated
      • GR, Ser134-phosphorylated
      • Nuclear receptor subfamily 3 group C member 1, Ser134-phosphorylated
      Background InformationGlucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes that control development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can represses gene transcriptions by blocking the nuclear translocation of transcription factors. BDNF-stimulated TrkB activation is shown to induce GR phosphorylation at Ser155 and Ser287 in primary rat cortical neurons (equivalent to human GR Ser134 and Ser267), resulting in enhanced GR transcription activity. GR is composed of an N-terminal modulating domain (a.a. 1-420), a DNA-binding domain (a.a. 421-486), and a C-terminal ligand-binding domain (a.a. 528-777).
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in Tris-buffered saline (pH 8.0) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of endogenous GR in primary rat cortical neurons (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).
      Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of exogenously expressed wild-type, but not S155A mutant, rat GR in transfected HEK293TrkB cells (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).
      Biological Information
      ImmunogenLinear peptide corresponding to a human glucocorticoid receptor sequence containing phosphorylated Ser134.
      EpitopepSer134.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThe target phosphorylation site recognized by this polyclonal antibody is present in all 9 human GR spliced isoforms (Ser108 of isoforms Alpha-B and Beta-B; Ser134 of all the other isoforms) and in both rat GR spliced isoforms (Ser155 of isoform A and Ser128 of isoform B) reported by UniProt (P04150 and P06536, respectively).
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat. Predicted to react with Canine, Horse, Feline, Sheep based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NR3C1
      • GCCR
      • GCR
      • GCRST
      • GRL
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~85 kDa observed. 87.56 kDa (Rat isoforms A), 84.83 kDa (Rat isoforms B), 85.66 kDa (Human isoform Alpha; Alpha-A), 81.51 kDa (Human isoform Beta; Beta-A), 85.82 kDa (Human isoform Alpha-2; Gamma), 81.67 kDa (Human isoform Beta-2), 64.75 kDa (Human isoform GR-A alpha), 60.60 kDa (Human isoform GR-A beta), 82.85 kDa (Human isoform Alpha-B), 78.70 kDa (Human isoform Beta-B), 82.90 kDa (Human isoform 10; hGRDelta313-338) calculated. Uncharacterized band(s) may appear in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in cell lysate from HEK293 expressing FLAG-tagged human glucocorticoid receptor.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected Ser134 phosphorylation of FLAG-tagged human glucocorticoid receptor in 10 ug lysate from BDNF-treated HEK293 transfectants.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1008 04055977334234