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OP64F Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF

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OP64F
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
      Catalogue NumberOP64F
      Brand Family Calbiochem®
      SynonymsAnti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
      References
      ReferencesAgarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889.
      Deng, C., et al. 1995. Cell 82, 675.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301.
      El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169.
      Li, R., et al. 1994. Nature 371, 534.
      Michieli, P., et al. 1994. Cancer Res. 54, 3391.
      Noda, A., et al.1994. Exp. Cell Res. 211, 90.
      El-Deiry, W.S., et al.1993. Cell 75, 817.
      Gu, Y., et al. 1993. Nature 366, 707.
      Harper, J.W., et al.1993. Cell 75, 805.
      Xiong, Y., et al.1993. Genes Devel. 7, 1572.
      Xiong, Y., et al.1993. Nature 366, 701.
      Xiong, Y., et al.1992. Cell 71, 505.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Negative controlUnstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
      Positive controlAny cell line expressing wild-type p53 (e.g. Hs27 cells or U2OS cells treated with DNA damaging agents) or UV-treated skin or colon tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections, Frozen Sections, Original Clone El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Epitope Identification Patrick O'Connor (NCI, personal communication).
      Key Applications Flow Cytometry
      Immunofluorescence
      Application NotesFlow Cytometry (2 µg/ml)
      Frozen Sections (5 µg/ml)
      Immunoblotting (use Cat. No. OP64)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (use Cat. No. OP64)
      Paraffin Sections (5 µg/ml, see comments)
      Application CommentsMaximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates p21WAF1 expression. Serum stimulation of quiescent cells will give low level p21WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can also be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. For staining paraffin sections, no pretreatment is usually needed, but some tissues may stain better after pretreating with heat or pressure cooker. In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. For immunoprecipitation and immunoblotting use Cat. No. OP64. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human p21WAF1
      ImmunogenHuman
      Epitopewithin amino acids 58-77 of human p21WAF1
      CloneEA10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      OP64F 0