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07-213 Anti-dimethyl-Histone H4 (Arg3) Antibody

07-213
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, InvWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H4 (Arg3) Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      Product Information
      FormatAffinity Purified
      Control
      • Acid extracted proteins from HeLa cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, 5 mg/mL BSA and 30% glycerol.
      Applications
      ApplicationAnti-dimethyl-Histone H4 (Arg3) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H4 (Arg3) also known as H4 histone family member A, histone 1 H4a & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesMethylation/Western Blot Assay: Reported by an independent laboratory to detect Histone H4 methylated in vitro by recombinant PRMT1 and detected by western blot analysis.
      Biological Information
      ImmunogenBSA-conjugated synthetic peptide (SG[me2R]GKGGKG-C) corresponding to amino acids 1-9 of Histone H4 with a C-terminal cysteine added to facilitate conjugation. The immunizing sequence is conserved from Tetrahymena to human.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes methylated Histone H4 (Arg3), MW 10 kDa. Addition of 0.1% Tween®-20 in western blot buffers results in the detection of additional proteins at 17 kDa (possibly methylated Histone H2a), 34 kDa and 80 kDa. Specificity demonstrated by ELISA using methylated and unmethylated Histone H4 peptides. The antibody does not detect recombinant Histone H3 purified from E. coli.
      IsotypeIgG
      Species Reactivity
      • Human
      • Vertebrates
      • Invertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Methylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: A 1:500-1:2000 dilution of this antibody detected methylated Histone H4 in acid-extracted proteins from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      07-213 04053252331459