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MABE651 Anti-dimethyl Histone H3 (Lys14), clone RM165 Antibody

This Anti-dimethyl Histone H3 (Lys14), clone RM165 Antibody is validated for use in Western Blotting, Multiplexing for the detection of dimethyl Histone H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B UniProt Number: P84243
MABE651
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, MplexRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE651
      DescriptionAnti-dimethyl Histone H3 (Lys14), clone RM165 Antibody
      Alternate Names
      • Histone H3.3
      • Histone H3
      Background InformationHistone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
      References
      Product Information
      FormatPurified
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide
      Quality LevelMQ300
      Applications
      ApplicationThis Anti-dimethyl Histone H3 (Lys14), clone RM165 Antibody is validated for use in Western Blotting, Multiplexing for the detection of dimethyl Histone H3.
      Key Applications
      • Western Blotting
      • Multiplexing
      Application NotesMultiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
      Biological Information
      ImmunogenA linear peptide corresponding to human Histone H3 dimethylated at Lys14.
      CloneRM165
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      • PP781
      • H3F3B
      • H3.3B
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in recombinant HeLa protein and HeLa acid extract.

      Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected dimethyl Histone H3 (Lys14) in HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE651 04055977181524

      Documentation

      Anti-dimethyl Histone H3 (Lys14), clone RM165 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-dimethyl Histone H3 (Lys14), clone RM165 Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl Histone H3 (Lys14), clone RM165 -QVP1503220 QVP1503220
      Anti-dimethyl Histone H3 (Lys14), clone RM165 Monoclonal Antibody VP1706194

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.