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MABS1916 Anti-c-Met Antibody, clone seeMet 13

Detect Hepatocyte growth factor receptor using this mouse monoclonal Anti-c-Met, clone seeMet 13, Cat. No. MABS1916, validated for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Inhibition studies.

Anti-c-Met Antibody, clone seeMet 13 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: MET, AUTS9, HCC, RCCP UniProt Number: P08581
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MABS1916
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, ICC, FC, InhibitionMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1916
      DescriptionAnti-c-Met Antibody, clone seeMet 13
      Alternate Names
      • Hepatocyte growth factor receptor
      • HGF receptor
      • HGF/SF receptor
      • Proto-oncogene c-Met
      • Scatter factor receptor
      • SF receptor
      • Tyrosine-protein kinase Met
      Background InformationHepatocyte growth factor receptor (EC 2.7.10.1; UniProt P08581; also known as HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met) is encoded by the MET (also known as AUTS9, HCC, RCCP) gene (Gene ID 4233) in human. HGF receptor or c-Met is initially produced as a 1390-a.a. precursor protein with an N-terminal signal peptide, postranslational proteolytic cleavage yields the mature tyrosine kinase receptor composed of an extracellular alpha chain (a.a. 25-307) linked via a disulfide bond to a transmembrane beta chain with an extracellular domain (a.a. 308-932), transmembrane helix (a.a. 933-955), and a cytoplasmic domain (a.a. 956-1390). The alpha-chain and the extracellular portion of beta-chain form the Sema domain that mediates receptor dimerization and ligand binding. The cytoplasmic portion of beta-chain contains the kinase domain (a.a. 1078-1345) and a C-terminal tail that contains a docking region (a.a. 1212-1390) for effector and adapter proteins essential for downstream signaling. Hepatocyte growth factor (HGF) is the only known c-Met ligand. HGF binding induces c-Met dimerization and autophosphorylation on Y1230, Y1234 and Y1235, leading to a conformation change and further phosphorylation on Y1349 and Y1356, making C-terminal tail docking region available for adaptor and signalling molecules. c-Met mediates a wide-range of biological activities, including cell proliferation, motility, angiogenesis and morphogenesis, and is an attractive target for cancer therapy. Aberrant c-Met activation/signalling promotes cancer cell proliferation and is associated with poor prognosis in various human cancers. Upregulated c-Met expression is also known to cause resistance against HER2, EGFR and B-RAF inhibitory drugs in cancer treatment.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationDetect Hepatocyte growth factor receptor using this mouse monoclonal Anti-c-Met, clone seeMet 13, Cat. No. MABS1916, validated for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Inhibition studies.
      Key Applications
      • Immunoprecipitation
      • Immunocytochemistry
      • Flow Cytometry
      • Inhibition
      Application NotesFlow Cytometry Analysis: 0.2 µL from a representative lot detected c-Met immunoreactivity on the surface of one million SNU-5 cells.

      Flow Cytometry Analysis: A representative lot immunostained live SNU-5 cells. A slight decrease in antibody immunoreactivity was observed when the temperature was dropped from 37°C to 4°C (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).

      Immunocytochemistry Analysis: A representative lot immunostained live SNU-5 cells. Indirect fluorescence labelling following subsequent cell fixation and permeabilization revealed increased antibody cytoplasmic internalization when antibody incubation was performed at 37°C than at 4°C (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated c-Met from SNU-5 cell lysate (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).

      Inhibition Analysis: A representative lot prevented HGF-induced scatter of HaCaT cells. Clone seeMet 13 inhibited cell proliferation by blocking cell division without inducing apoptosis (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).

      Note: seeMet 13 exhibited low reactivity and specificity towards denatured c-Met by Western blotting. This monoclonal antibody is not recommended for Western blotting application (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
      Biological Information
      ImmunogenHis-tagged recombinant human c-Met alpha chain (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
      CloneseeMet 13
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone seeMet 13 bound strongly to native c-Met by flow cytometry, while exhibiting low reactivity and specificity towards denatured c-Met by Western blotting (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MET
      • AUTS9
      • HCC
      • RCCP
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight32.51 kDa (alpha chain; a.a. 25-307), 155.5/153.0 kDa (pro-/mature uncleaved isoform 1), 157.7/155.2 kDa (pro-/mature uncleaved isoform 2), 85.75/83.25 kDa (pro-/mature uncleaved isoform 3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in SNU-5 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected both surface and internalized c-Met by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized SNU-5 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1916 04054839090233