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16-210 Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate

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16-210
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RFC, ICC, WBMFITCMonoclonal Antibody
      Description
      Catalogue Number16-210
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate
      OverviewNote: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals

      Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)

      Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254
      Alternate Names
      • 70 kDa zeta-associated protein
      • Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD)
      • zeta-chain (TCR) associated protein kinase 70kDa
      • zeta-chain associated protein kinase 70kDa
      • zeta-chain associated protein kinase, 70kD
      Background InformationZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
      Product Information
      FormatFITC
      Control
      • Western Blot: Jurkat cell lysate.
      PresentationMouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide.

      Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.
      Biological Information
      ImmunogenGST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.
      Cloneclone 2F3.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes human ZAP-70.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman. Expected to cross-react with rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
      Gene Symbol
      • ZAP70
      • ZAP-70
      • TZK
      • STD
      • SRK
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
      SIZE: 619 amino acids; 69872 Da
      SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
      TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
      DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
      PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
      DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
      Molecular Weight70 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Jurkat lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      16-210 04053252336331