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ABN1687-100UG Anti-TBR-2

Anti-TBR-2, Cat. No. ABN1687, is a highly specific rabbit polyclonal antibody that targets Eomesodermin homolog (Tbr2) and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.

Anti-TBR-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Eomes, Tbr2 UniProt Number: O54839
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ABN1687-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIH(P), IF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1687-100UG
      DescriptionAnti-TBR-2
      Alternate Names
      • Eomesodermin homolog
      • T-box brain protein 2
      • T-brain-2
      Background InformationEomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. TBR-2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of TBR-2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of TBR-2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
      Applications
      ApplicationAnti-TBR-2, Cat. No. ABN1687, is a highly specific rabbit polyclonal antibody that targets Eomesodermin homolog (Tbr2) and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected TBR-2 in Immunofluorescence applications (Nelson, B.R., et. al. (2013). J Neurosci. 33(21):9122-39; Hodge, R.D., et. al. (2013). J Neurosci. 33(9):4165-80).

      Immunohistochemistry Analysis: A representative lot detected TBR-2 in Immunohistochemistry applications (Yoon, K.J., et. al. (2008). Neuron. 58(4):519-31).

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TBR-2 in mouse hippocampus, embryonic mouse brain, and embryonic mouse gut tissue sections.
      Biological Information
      ImmunogenConjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine TBR-2.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects T-box brain protein 2 (TBR-2) in murine species. It targets an epitope with in 20 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Eomes
      • Tbr2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~63 kDa observed; 74.80 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse E13-14 brain.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected TBR-2 in mouse E13-14 brain.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1687-100UG 04054839531859