Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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ST1718
Sigma-AldrichAnti-SMO Mouse mAb (1D9)
This Anti-SMO Mouse mAb (1D9) is validated for use in Immunoblotting for the detection of SMO.
More>>This Anti-SMO Mouse mAb (1D9) is validated for use in Immunoblotting for the detection of SMO. Less<<
Anti-SMO Mouse mAb (1D9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~75 kDa SMO protein in A431 cells.
Catalogue Number
ST1718
Brand Family
Calbiochem®
Synonyms
Anti-Smoothened
Application Data
Detection of human SMO by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-SMO Mouse mAb (1D9) (Cat. No. ST1718) (1-5 µg/ml). Detection: chemiluminescence.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 JLN
Synonyms
Anti-Smoothened
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human SMO by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-SMO Mouse mAb (1D9) (Cat. No. ST1718) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~75 kDa SMO protein.
Background
SMO is a G protein-coupled receptor that probably associates with the protein, Patched (PTCH), to transduce hedgehog signalling. Binding of sonic hedgehog (SHH) to its receptor, Patched, is thought to inhibit the interaction between SMO and Patched.
Host
Mouse
Immunogen
Full-length, recombinant, human SMO (aa 653-787) expressed as a GST fusion protein
Clone
1D9
Isotype
IgG2a
Species
human
Positive control
A431 cells
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
