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05-389-AF647 Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: RAC1, TC25, MIG5 UniProt Number: P63000
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05-389-AF647
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-389-AF647
      DescriptionAnti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Ras-related C3 botulinum toxin substrate 1
      • Cell migration-inducing gene 5 protein
      • Ras-like protein TC25
      • p21-Rac1
      • Rac1
      Background InformationRas-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
      Biological Information
      ImmunogenRecombinant protein containing the full length human Rac.
      Clone23A8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
      IsotypeIgG2b
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAC1
      • TC25
      • MIG5
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~21 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      05-389-AF647 04055977294910