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ABN38 Anti-RAB35 Antibody

This Anti-RAB35 Antibody is validated for use in WB, IH(P) for the detection of RAB35.

Anti-RAB35 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: RAB35, RAB1C, RAY UniProt Number: Q15286
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ABN38
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Ca, EqWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN38
      DescriptionAnti-RAB35 Antibody
      Alternate Names
      • Ras-related protein Rab-35
      • GTP-binding protein RAY
      • Ras-related protein Rab-1C
      Background InformationRAB35 is a member of the Rab GTPases identified in the human genome. It is ubiquitously expressed and regulates a fast endocytotic recycling events associated with the terminal steps of cytokinesis, the formation of immunological synapse, and peptide loading onto major histocompatibility complex III molecules. RAB35 also plays a significant role in the formation of the immunological synapse (IS) between peptide-loaded antigen presenting cells (APCs) and T cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human brain tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-RAB35 Antibody is validated for use in WB, IH(P) for the detection of RAB35.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RAB35 in normal human brain tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human RAB35.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes RAB35 at the C-terminus.
      Species Reactivity
      • Human
      • Rat
      • Canine
      • Horse
      Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Canine and Equine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAB35
      • RAB1C
      • RAY
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.

      SUBUNIT STRUCTURE: Interacts with DENND1A and DENND1B in a nucleotide-dependent manner. The low binding to DENND1C is nucleotide-independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein.

      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Membrane › clathrin-coated pit. Cytoplasmic vesicle › clathrin-coated vesicle. Endosome. Melanosome. Note: Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Rab family.
      Molecular Weight~19 kDa observed. The calculated molecular weight is 23 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human brain tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected RAB35 on 10 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN38 04053252423703

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-RAB35 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-RAB35 Antibody Certificates of Analysis

      TitleLot Number
      Anti-RAB35 - 2512191 2512191
      Anti-RAB35 - 2188562 2188562
      Anti-RAB35 - 2196091 2196091
      Anti-RAB35 - 3474147 3474147
      Anti-RAB35 - NRG1929409 NRG1929409
      Anti-RAB35 -2765140 2765140
      Anti-RAB35 -2767213 2767213
      Anti-RAB35 Polyclonal Antibody 3170231
      Anti-RAB35 Polyclonal Antibody 2967240
      Anti-RAB35 Polyclonal Antibody 3089162