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05-719 Anti-Phosphatidylserine Antibody, clone 1H6

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05-719
200 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtFC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-719
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphatidylserine Antibody, clone 1H6
      OverviewSignificance:
      Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
      Background InformationPhosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified IgG supplied in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 before the addition of glycerol to 30%. Liquid at -20°C.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphatidylserine Antibody, clone 1H6 is an antibody against Phosphatidylserine for use in FC, IH.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesFlow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.

      Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
      Biological Information
      ImmunogenLiposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol
      Cloneclone 1H6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes phosphatidylserine (PS) in cell membranes
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      Molecular Weightnone given
      Product Usage Statements
      Quality Assuranceroutinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-719 04053252670220