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05-718 Anti-PML Antibody, clone 36.1-104

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05-718
200 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-718
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PML Antibody, clone 36.1-104
      Background InformationPromyeloctic Leukemia Gene product (PML) is a nuclear protein with growth-suppressive properties originally identified in the context of the PML-retinoic receptor alpha (RAR alpha) fusion protein of acute promyelocytic leukemia. PML localizes within distinct nuclear structures, called nuclear bodies, which are disrupted by the expression of PML-RAR alpha.
      Product Information
      FormatPurified
      Control
      • MEF1 cell lysate
      PresentationProtein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect PML also known as Promyelocytic Leukemia Protein with Anti-PML Antibody, clone 36.1-104 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      Immunogen6His fusion protein corresponding to mouse PML (promyelocytic leukemia protein)
      Clone36.1-104
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes PML.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • PML
      • PP8675
      • RNF71
      • TRIM19
      • MYL
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29590 # Probable transcription factor. May play an important role in recruitment of ELF4 into PML nuclear bodies.
      SIZE: 882 amino acids; 97517 Da
      SUBUNIT: Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Interacts with the C-terminus of ELF4. Interacts with Lassa virus Z protein and rabies virus phosphoprotein (By similarity).
      SUBCELLULAR LOCATION: Nucleus. Note=Localized to PML nuclear bodies.
      PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
      DISEASE: SwissProt: P29590 # A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
      SIMILARITY: SwissProt: P29590 ## Contains 2 B box-type zinc fingers. & Contains 1 RING-type zinc finger.
      Molecular Weight90-106 kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse embryo fibroblast (MEF1) cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-718 04053252333743