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OP74 Anti-PKC (Ab-2) Mouse mAb (MC5)

OP74
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~79-80 kDa protein kinase C α/β1/β2 isoforms in NIH3T3 cells and brain tissue.

      Catalogue NumberOP74
      Brand Family Calbiochem®
      SynonymsAnti-Protein Kinase C
      References
      ReferencesXiao, H., et al. 1994. J. Neurosurg. 81, 734.
      Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
      Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
      Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
      Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
      Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
      Nishizuka, Y., 1989. Cancer 63, 1892.
      Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Positive controlNIH3T3 cells or brain tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunoprecipitation Yong, S., et al. 1988. Eur. J. Biochem. 173, 247. Original Clone Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesBlocking Studies (5 µg/ml, see comments)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (see application references)
      Paraffin Sections (5 µg/ml, no pre-treatment required)
      Application CommentsMay be used to block the down regulation of PKC activity. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified bovine brain PKC
      ImmunogenBovine
      Epitopewithin the hinge region of PKC
      CloneMC5
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      OP74 0