MAB345A4
Anti-O4 Antibody, clone 81, Alexa Fluor™488 Conjugate
clone 81 (mAB 04), from mouse, ALEXA FLUOR™ 488
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
81 (mAB 04), monoclonal
Application:
ICC
Citations:
2
biological source
mouse
Quality Segment
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
81 (mAB 04), monoclonal
species reactivity
rat
species reactivity (predicted by homology)
human (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
General description
Oligodendrocytes and astrocytes are derived from common precursor cells, glioblasts (Sommer, 1981). O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatally (Schachner, 1981) and is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in pro-oligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain. Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments.
Immunogen
Homogenate of white matter of corpus callosum from bovine brain.
Application
Anti-O4 Antibody, clone 81, Alexa Fluor™488 Conjugate detects level of O4 & has been published & validated for use in ICC.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Biochem/physiol Actions
This antibody recognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm.
Physical form
Precipitation followed by ion exchange purification
Purified mouse monoclonal IgM conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Preparation Note
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Undifferentiated rat oligodendrocyte precursor cells
Undifferentiated rat oligodendrocyte precursor cells
Evaluated by Immunocytochemistry in undifferentiated rat oligodendrocyte precursor cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected O4 in undifferentiated rat oligodendrocyte precursor cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected O4 in undifferentiated rat oligodendrocyte precursor cells.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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