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MAB4100 Anti-MRP1 Antibody, clone QCRL-1

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MAB4100
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, WB, ICC, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MRP1 Antibody, clone QCRL-1
      Alternate Names
      • Multidrug Resistance Protein-1
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).

      Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).

      Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).

      Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).

      Immunoprecipitation (Hipfner, et al., 1994; 1996).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenNon-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
      CloneQCRL-1
      HostMouse
      SpecificityReacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane

      phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines

      and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,

      MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternative splicing by exon deletion results in several splice variants but maintains the original open reading frame in all forms.
      Gene Symbol
      • ABCC1
      • 4transporter)
      • ABC29
      • GS-X
      • ABCC
      • MRP1
      • DKFZp781G125
      • MRP
      • DKFZp686N04233
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P33527 # May participate directly in the active transport of drugs into subcellular organelles or influence drug distribution indirectly. Confers resistance to anticancer drugs. Transports LTC4. May protect milk against xenobiotics (By similarity).
      SIZE: 1531 amino acids; 171561 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Lung, testis and peripheral blood mononuclear cells.
      SIMILARITY: SwissProt: P33527 ## Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4100 04053252666551