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MAB3418-25UG Anti-MAP-2, clone AP20

Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.

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MAB3418-25UG
25 μg  
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      Description
      Catalogue NumberMAB3418-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MAP-2, clone AP20
      Alternate Names
      • Microtubule Associated Protein
      • MAP2A and 2B
      Background InformationMAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
      References
      Product Information
      FormatPurified
      Control
      • Brain, Neuronal culture, Human glioblastoma T98G cells.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot:
      60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.

      Immunohistochemistry:
      5 µg/mL (4% PFA fixed frozen sections).

      Immunohistochemistry:
      5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

      Optimal working concentrations must be determined by the end user.
      Biological Information
      ImmunogenBovine brain microtubule protein.
      CloneAP20
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityMAB3418 binds specifically to MAP-2.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Xenopus
      • Quail
      Species Reactivity NoteRat and mouse. Expected to cross-react with human, cow, chicken, quail, and Xenopus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
      Gene Symbol
      • MAP2
      • MAP2B
      • MAP-2
      • MAP2C
      • DKFZp686I2148
      • MAP2A
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
      SIZE: 1827 amino acids; 199539 Da
      PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
      Molecular Weight230 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Rat Brain lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3418-25UG 04054839337468

      Documentation

      Anti-MAP-2, clone AP20 Certificates of Analysis

      TitleLot Number
      Anti-MAP-2, clone AP20 - 3173528 3173528
      Anti-MAP-2, clone AP20 - 3221956 3221956
      Anti-MAP-2, clone AP20 - 3321793 3321793
      Anti-MAP-2, clone AP20 - 3474129 3474129
      Anti-MAP-2, clone AP20 - 3760532 3760532
      Anti-MAP-2, clone AP20 - 3787023 3787023
      Anti-MAP-2, clone AP20 - 3809265 3809265
      Anti-MAP-2, clone AP20 - 3841827 3841827
      Anti-MAP-2, clone AP20 - 3849131 3849131
      Anti-MAP-2, clone AP20 - 3872434 3872434
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