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MABN2408-25UL Anti-KCNK16 (TALK1) Antibody, clone N466/14

Anti-KCNK16 (TALK1), clone N466/14, Cat. No. MABN2408, is a mouse monoclonal antibody that detects Potassium channel, subfamily K, member 16 (TALK1) and is tested for use in Immunohistochemistry (Para

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MABN2408-25UL
25 µL  
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      Overview

      Description
      Catalogue NumberMABN2408-25UL
      DescriptionAnti-KCNK16 (TALK1) Antibody, clone N466/14
      Alternate Names
      • TALK1
      • Potassium channel
      • subfamily K member 16
      Background InformationPotassium channel, subfamily K, member 16 (UniProt: G5E845; also known as KCNK16, TALK1) is encoded by the Kcnk16 gene (Gene ID: 74571) in murine species. KCNK16 is a multi-pass membrane protein that contains two pore-forming P domains. It is an open rectifier that primarily passes outward current under physiological K+ concentrations. KCNK16 is predominantly expressed in the pancreas and is activated at alkaline pH. Mice deficient in KCNK16 display reduced food intake and elevated oxygen consumption and energy expenditure. Islets with Kcnk -/- show enhanced insulin secretion and GIP-1 stimulation. KCNK16 is also reported to reduce delta-cell cytosolic Ca2+ elevations and somatostatin release by limiting their Ca2+-induced Ca2+ release and can modulate the intra islet paracrine signaling mechanisms that control glucagon secretion. KCNK16 is also reported to regulate beta-cell endoplasmic reticulum (ER) Ca2+ handling by conducting K+ counter currents across the ER membrane that promotes ER Ca2+ leak. Inhibition of KCNK16 channel activity is shown to augment ER Ca2+ stores. (Ref.: Brachs, S., et al. (2019). Diabetes 68 (suppl. 1); 2492; Vierra, NC., et al. (2018). Mol. Metab. 9; 84-97).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-KCNK16 (TALK1), clone N466/14, Cat. No. MABN2408, is a mouse monoclonal antibody that detects Potassium channel, subfamily K, member 16 (TALK1) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected KCNK16 (TALK1) in rat brain and mouse brain tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFusion protein fragment corresponding to 53 amino acids from the first extracellular loop and 33 amino acids from the cytoplasmic C-terminus of mouse Potassium channel subfamily K member 16 (KCNK16).
      EpitopeUnknown
      CloneN466/14
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone N466/14 is a mouse monoclonal antibody that detects Potassium channel subfamily K member 16 (KCNK16).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kcnk16
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight53 kDa observed; 32.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse pancreas tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KCNK16 (TALK1) in Mouse pancreas tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2408-25UL 04065265237710