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MABF2218-25UG Anti-Influenza A Virus NSP1 Antibody, clone 2H6

Anti-Influenza A Virus NSP1, clone 2H6, Cat. No. MABF2218, is a mouse monoclonal antibody that detects NS1 protein and is tested for use in ELISA, Immunofluorescence, Inhibition Assay, Neutralizing, Surface plasmon resonance, and Western Blotting.

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MABF2218-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2218-25UG
      DescriptionAnti-Influenza A Virus NSP1 Antibody, clone 2H6
      Alternate Names
      • NS1
      Background InformationNon-structural protein 1 (UniProt: Q4QZ06; also known as NS1) is encoded by the NS1 (also known as NS) gene (Gene ID: 956533) in Influenza A virus. Influenza A virus (IAV) is a highly contagious member of Orthomyxoviridae family. The non-structural protein NS1 is a well conserved protein that is expressed at high levels in infected cells. It is a multifunctional protein associated with various viral functions including mRNA processing, inhibition of cellular apoptosis, limiting interferon production, and inhibition of mRNA splicing. NS1 has two functionally distinct domains: the N-terminal RNA binding domain (RBD) that consists of three -helices; and the C-terminal effector domain that consists of seven -strands and three -helices. The RBD domain binds with several RNA species with low affinity and the effector domain predominantly interacts with host-cell proteins and stabilizes the RBD domain. Clone 2H6 recognizes multiple strains of H5N1 influenza virus. It exhibits high affinity for the RBD of NS1 protein (Kd = 43.5 nM). Threonine 49 (T49) residue in NS1 that forms a direct hydrogen bond with the double stranded RNA is reported to be critical in its interaction with 2H6 antibody. This antibody also reacts with NS1 expressed in virus infected MDCK cells. (Ref.: Barnwal, B., et al. (2015). Antiviral Res. 116; 55-61; Tan, Z., et al. (2010). J. Med. Virol. 82(3); 467-475).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Influenza A Virus NSP1, clone 2H6, Cat. No. MABF2218, is a mouse monoclonal antibody that detects NS1 protein and is tested for use in ELISA, Immunofluorescence, Inhibition Assay, Neutralizing, Surface plasmon resonance, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Inhibition
      • Neutralizing
      • Surface plasmon resonance
      • Western Blotting
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected Influenza A Virus NS1 protein in Immunofluorescence applications. (Tan, Z., et al. (2010). J Med Virol. 82(3):467-75; Wu, J., et al. (2016). Sci Rep.;6:33382).

      Surface plasmon resonance: A representative lot detected Influenza A Virus NS1 protein in Surface plasmon resonance applications. (Barnwal, B., et al. (2015). Antiviral Res. 116:55-61).

      Western Blotting Analysis: A representative lot detected Influenza A Virus NS1 protein in Western Blotting applications (Tan, Z., et al. (2010). J Med Virol. 82(3):467-75; Wu, J., et al. (2016). Sci Rep. 6:33382).

      Neutralizing: A representative lot of this antibody bound NS1 and reduced influenza A virus replication. (Barnwal, B., et al. (2015). Antiviral Res. 116: 55-61).

      ELISA Analysis: A representative lot detected Influenza A Virus NSP1 in ELISA applications (Barnwal, B., et al. (2015). Antiviral Res. 116:55-61; Wu, J., et al. (2016). Sci Rep. 6: 33382).

      Inhibition Assay: A representative lot of this antibody inhibited the interaction between NS1 and dsRNA. (Wu, J., et al. (2016). Sci Rep. 6:33382).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged full-length recombinant NS1 protein of Influenza A virus expressed in E.coli.
      EpitopeN-terminal
      Clone2H6
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2H6 is a mouse monoclonal antibody that detects non-structural protein 1 (NS1) of the Influenza A virus. It shows reactivity with H1N1, H3N2, and H5N1 strains.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteInfluenza A Virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • NS1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed for the recombinant NS1 protein; 25.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Influenza A virus non-structural (NS1) protein .

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Influenza A Virus NS1 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2218-25UG 04065267403939