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CA1031 Anti-ICMT (170-279) Rabbit pAb

Anti-ICMT (170-279) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Isoprenylcysteine carboxyl methyltransferase

CA1031
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~32 kDa human ICMT protein in ICMT-transfected Sf9 insect cells.
      Catalogue NumberCA1031
      Brand Family Calbiochem®
      SynonymsAnti-Isoprenylcysteine carboxyl methyltransferase
      Application Data
      Detection of human ICMT by immunoblotting. Samples: whole cell lysate (lanes 1-3) (30 µg, 10 µg, and 3 µg, respectively) containing full-length ICMT from transfected Sf9 cells, whole cell lysate from untransfected Sf9 cells (lane 4), whole cell lysate containing the 12.9 kDa immunogen (lanes 5-7) (0.01 µg, 0.03 µg, and 0.1 µg, respectively) from transfected Sf9 cells. Primary antibody: Anti-ICMT (170-279) Rabbit pAb (Cat. No. CA1031) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesAnderson, J.L., et al. 2005. J.Biol. Chem. 280, 29454.
      Winter-Vann, S.G., et al. 2005. PNAS USA 102, 4336.
      Product Information
      FormLiquid
      FormulationIn 20 mM PBS.
      Positive controlICMT-transfected Sf9 cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.5-1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 170-279 of human ICMT, expressed in Sf9 cells
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CA1031 0

      Documentation

      References

      Reference overview
      Anderson, J.L., et al. 2005. J.Biol. Chem. 280, 29454.
      Winter-Vann, S.G., et al. 2005. PNAS USA 102, 4336.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-Isoprenylcysteine carboxyl methyltransferase
      ApplicationImmunoblotting (0.5-1 µg/ml)
      Application Data
      Detection of human ICMT by immunoblotting. Samples: whole cell lysate (lanes 1-3) (30 µg, 10 µg, and 3 µg, respectively) containing full-length ICMT from transfected Sf9 cells, whole cell lysate from untransfected Sf9 cells (lane 4), whole cell lysate containing the 12.9 kDa immunogen (lanes 5-7) (0.01 µg, 0.03 µg, and 0.1 µg, respectively) from transfected Sf9 cells. Primary antibody: Anti-ICMT (170-279) Rabbit pAb (Cat. No. CA1031) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~32 kDa ICMT protein.
      BackgroundIsoprenylcysteine methyl transferase (ICMT) is an enzyme that catalyzes the transfer of methyl groups to the C-terminal prenylated cysteine in Ras family proteins and other related signaling proteins. This methylation is necessary for proper membrane targeting and transforming capability of Ras. Small molecule inhibition of ICMT results in mislocalization of Ras, imparied signaling through Ras pathways, and blocking of anchorage-independent growth in specific colon cancer cell lines.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 170-279 of human ICMT, expressed in Sf9 cells
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlICMT-transfected Sf9 cells
      FormLiquid
      FormulationIn 20 mM PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Regulatory Review
      ReferencesAnderson, J.L., et al. 2005. J.Biol. Chem. 280, 29454.
      Winter-Vann, S.G., et al. 2005. PNAS USA 102, 4336.