Millipore Sigma Vibrant Logo

AB3080 Anti-Green Fluorescent Protein Antibody

View Products on Sigmaaldrich.com
AB3080
50 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtELISA, ICC, IHC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3080
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Green Fluorescent Protein Antibody
      Alternate Names
      • GFP
      • eGFP
      Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
      Product Information
      FormatPurified
      Control
      • GFP fusion protein expressed in cells
      • RENCX cells
      PresentationPurified rabbit polyclonal IgG in 10 mM Tris buffer, pH 7.2 with 0.01% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Green Fluorescent Protein Antibody is validated for use in ELISA, IC, IH, WB for the detection of Green Fluorescent Protein.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot:
      A previous lot of this antibody was used in western blot.

      ELISA:
      A 1:2000-1:10,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay.

      Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore’s highly-purified secondary antibodies in indirect detection systems.

      Immunocytochemistry:

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHighly purified native GFP from Aequorea victoria.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
      Species Reactivity
      • Vertebrates
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GFP
      • eGFP
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular WeightGFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunocytochemistry on RENCX cells.

      Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot of anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB3080 04053252743511