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PC246 Anti-GluR1 Rabbit pAb

Anti-GluR1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glutamate Receptor I

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PC246
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~105-107 kDa GluR1 protein in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain extracts.
      Catalogue NumberPC246
      Brand Family Calbiochem®
      SynonymsAnti-Glutamate Receptor I
      References
      ReferencesZuo, J. et al. 1997. Nature 388, 769.
      McHugh, T.J., et al. 1996. Cell 87, 1339.
      Tsien, J.Z., et al. 1996. Cell 87, 1327.
      Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology 44, S7.
      Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci. 17, 31.
      Bliss, T.V.P. and Collingridge, G.L. 1993. Nature 361, 31.
      Lomeli, H., et al. 1993. FEBS Lett. 315, 318.
      Molnar, E. et al. 1993. Neuroscience 53, 307.
      Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev. 16, 13.
      Beal, M.F. 1992. FASEB J. 6, 3338.
      Rothman, S.M. 1992. Ann. NY Acad. Sci. 648, 132.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlD425 Med cells, hippocampal neurons
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunofluorescence Cottrell, J.R. 2004. Neuron 44, 677.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting 1-5 µg/ml)
      Immunofluorescence (10-20 µg/ml, see application references)
      Application CommentsAlso recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC246 0

      Documentation

      Anti-GluR1 Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC246

      References

      Reference overview
      Zuo, J. et al. 1997. Nature 388, 769.
      McHugh, T.J., et al. 1996. Cell 87, 1339.
      Tsien, J.Z., et al. 1996. Cell 87, 1327.
      Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology 44, S7.
      Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci. 17, 31.
      Bliss, T.V.P. and Collingridge, G.L. 1993. Nature 361, 31.
      Lomeli, H., et al. 1993. FEBS Lett. 315, 318.
      Molnar, E. et al. 1993. Neuroscience 53, 307.
      Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev. 16, 13.
      Beal, M.F. 1992. FASEB J. 6, 3338.
      Rothman, S.M. 1992. Ann. NY Acad. Sci. 648, 132.

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-January-2011 JSW
      SynonymsAnti-Glutamate Receptor I
      ApplicationImmunoblotting 1-5 µg/ml)
      Immunofluorescence (10-20 µg/ml, see application references)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~105-107 kDa GluR1 protein.
      BackgroundGlutamate is the major excitatory neurotransmitter in the central nervous system. Precise regulation of glutamate levels are necessary as excess glutamate is toxic to neurons, presumably through receptor activation which leads to elevated intracellular Ca2+ levels. Multiple glutamate receptors have been identified in the mammalian brain, including the ligand-gated ion channels (ionotropic glutamate receptors) which are permeable to cations. These receptors have been classified into multiple subtypes based upon pharmacological and electrophysiological data, and includes the α-amino-3-hydroxy-5-methyl-4-isoxaxole propionate (AMPA)/kainate, and N-methyl-D-aspartate (NMDA) receptors. The ligand-gated glutamate receptors are multimeric heteromers composed of distinct subunits. Another subfamily of glutamate receptors includes eight G protein-coupled metabotropic glutamate receptors. The delta receptors are believed to represent another class of ionotropic glutamate receptors, although glutamate binding and ion channel activity remain to be demonstrated. Glutamate receptors likely play a key role in learning and memory, and it has been proposed that several neurodegenerative diseases may involve neural cell death caused by excessive activation of the glutamate receptors.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)
      IsotypeIgG
      Speciesrat
      Positive controlD425 Med cells, hippocampal neurons
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesZuo, J. et al. 1997. Nature 388, 769.
      McHugh, T.J., et al. 1996. Cell 87, 1339.
      Tsien, J.Z., et al. 1996. Cell 87, 1327.
      Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology 44, S7.
      Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci. 17, 31.
      Bliss, T.V.P. and Collingridge, G.L. 1993. Nature 361, 31.
      Lomeli, H., et al. 1993. FEBS Lett. 315, 318.
      Molnar, E. et al. 1993. Neuroscience 53, 307.
      Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev. 16, 13.
      Beal, M.F. 1992. FASEB J. 6, 3338.
      Rothman, S.M. 1992. Ann. NY Acad. Sci. 648, 132.
      Application referencesImmunofluorescence Cottrell, J.R. 2004. Neuron 44, 677.