Millipore Sigma Vibrant Logo

CP28 Anti-Fibroblast Antigen Mouse mAb (AS02)

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sectio

 More>>
Anti-Fibroblast Antigen Mouse mAb (AS02) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

View Products on Sigmaaldrich.com
CP28
  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MkMMonoclonal Antibody
      Description
      OverviewRecognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
      Catalogue NumberCP28
      Brand Family Calbiochem®
      SynonymsAnti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
      References
      ReferencesSaalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
      Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
      Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
      Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
      Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
      Product Information
      DeclarationManufactured by Dianova, GmbH, Germany.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 6% BSA.
      Positive controlAny fibroblast cell line or skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesSaalbach, A., et al. 2000. J. Invest. Dermatol. 115, 882. Saalbach, A., et al. 1999. Cell Tissue Res. 298, 307. Saalbach, A., et al. 1998. Arch. Dermatol. Res. 290, 360. Saalbach, A., et al. 1997. Cell Tissue Res. 290, 539. Saalbach, A., et al. 1996. J. Invest. Dermatol. 106, 1314.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFlow Cytometry (see comments)
      Frozen Sections (10 µg/ml, acetone fixation)
      Immunoblotting (10 µg/ml, non-reducing only)
      Immunofluorescence (10 µg/ml)
      Immunoprecipitation (2-10 µg/ml)
      Application CommentsThis antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman fibroblasts
      ImmunogenHuman
      CloneAS02
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      CP28 0