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CP28 Anti-Fibroblast Antigen Mouse mAb (AS02)

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).

Anti-Fibroblast Antigen Mouse mAb (AS02) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MkMMonoclonal Antibody
      Description
      OverviewRecognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
      Catalogue NumberCP28
      Brand Family Calbiochem®
      SynonymsAnti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
      References
      ReferencesSaalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
      Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
      Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
      Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
      Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
      Product Information
      DeclarationManufactured by Dianova, GmbH, Germany.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 6% BSA.
      Positive controlAny fibroblast cell line or skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesSaalbach, A., et al. 2000. J. Invest. Dermatol. 115, 882. Saalbach, A., et al. 1999. Cell Tissue Res. 298, 307. Saalbach, A., et al. 1998. Arch. Dermatol. Res. 290, 360. Saalbach, A., et al. 1997. Cell Tissue Res. 290, 539. Saalbach, A., et al. 1996. J. Invest. Dermatol. 106, 1314.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFlow Cytometry (see comments)
      Frozen Sections (10 µg/ml, acetone fixation)
      Immunoblotting (10 µg/ml, non-reducing only)
      Immunofluorescence (10 µg/ml)
      Immunoprecipitation (2-10 µg/ml)
      Application CommentsThis antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman fibroblasts
      ImmunogenHuman
      CloneAS02
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP28 0

      Documentation

      Anti-Fibroblast Antigen Mouse mAb (AS02) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fibroblast Antigen Mouse mAb (AS02) Certificates of Analysis

      TitleLot Number
      CP28

      References

      Reference overview
      Saalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
      Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
      Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
      Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
      Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2007 RFH
      SynonymsAnti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
      ApplicationFlow Cytometry (see comments)
      Frozen Sections (10 µg/ml, acetone fixation)
      Immunoblotting (10 µg/ml, non-reducing only)
      Immunofluorescence (10 µg/ml)
      Immunoprecipitation (2-10 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
      BackgroundThe ability to distinguish fibroblast contamination in cell cutures and tissues is an important biological problem. Identification of fibroblastic components of cells and tissues is also useful to determine the potential fibroblastic origin of tumors. The ability to specifically detect fibroblasts in tissue culture samples and in tissue samples in situ is often difficult. One of the chief problems has been the identification of a protein which is specific to fibroblasts and not shared by other cell types such as endothelial cells and chondrocytes. Most fibroblast specific reagents show some cross reactivity with monocytes/macrophages and smooth muscle cells. Currently, the most commonly used approach has been to use antibodies to proline-4-hydroxylase, a protein involved in the synthesis of collagen. However, proline-4-hydroxylase is present in all cells which make collagen, not just fibroblasts. Also, proline-4-hydroxylase is intracellular, which makes it less attractive to many as a marker, and most antibodies to proline-4-hydroxylase will not stain quiescent cells that are not actively producing collagen. CD90, or Thy-1 surface antigen, is restricted to the expression on extracellular membrane of fibroblast cells, neurons, and some CD34+ blood stem cells. This antibody shows no reactivity with keratinocytes, chondrocytes, smooth muscle, Langerhans, or endothelial cells in situ nor will it bind to extracellular matrix proteins. The antigen shows no change in levels of expression under different growth conditions including changes in serum levels, passage number of cells, etc. Interestingly, the level of CD90 expression increases in tissues during wound healing.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman fibroblasts
      CloneAS02
      IsotypeIgG₁
      Specieshuman, monkey, not mouse, not rat
      Positive controlAny fibroblast cell line or skin tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 6% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesSaalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
      Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
      Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
      Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
      Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
      Application referencesSaalbach, A., et al. 2000. J. Invest. Dermatol. 115, 882. Saalbach, A., et al. 1999. Cell Tissue Res. 298, 307. Saalbach, A., et al. 1998. Arch. Dermatol. Res. 290, 360. Saalbach, A., et al. 1997. Cell Tissue Res. 290, 539. Saalbach, A., et al. 1996. J. Invest. Dermatol. 106, 1314.