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ST1137 Anti-FOXP3 Mouse mAb (3G3)

Anti-FOXP3 Mouse mAb (3G3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1137
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~47 kDa FOXP3 protein in lymph node cells.
      Catalogue NumberST1137
      Brand Family Calbiochem®
      Application Data
      Detection of human FOXP3 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with full-length FOXP3 (lane 1) or mock-transfected (lane 2). Primary antibody: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (2 µg/ml). Detection: chemiluminescence.

      Detection of human FOXP3 by flow cytometry. Samples: mouse lymph node cells (2x106 cells). Primary antibodies: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (100 ng in 50 µl total volume) (upper panel) and Anti-CD4 control antibody (lower panel). Detection: fluorescence.
      References
      ReferencesGavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.05% BSA.
      Positive controlLymph node cells
      Preservative≤ 0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (2 µg/ml)
      Flow cytometry (100 ng/2 x 10⁶ cells in 50 µl total volume)
      Application CommentsAntibody should be titrated for optimal results in individual sytems.
      Biological Information
      Immunogenfull-length, recombinant, mouse FOXP3 containing a His•Tag® sequence
      ImmunogenMurine
      EpitopeWithin the N-terminus
      Clone3G3
      HostMouse
      IsotypeIgG1
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1137 0

      Documentation

      References

      Reference overview
      Gavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-July-2008 RFH
      ApplicationImmunoblotting (2 µg/ml)
      Flow cytometry (100 ng/2 x 10⁶ cells in 50 µl total volume)
      Application Data
      Detection of human FOXP3 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with full-length FOXP3 (lane 1) or mock-transfected (lane 2). Primary antibody: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (2 µg/ml). Detection: chemiluminescence.

      Detection of human FOXP3 by flow cytometry. Samples: mouse lymph node cells (2x106 cells). Primary antibodies: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (100 ng in 50 µl total volume) (upper panel) and Anti-CD4 control antibody (lower panel). Detection: fluorescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~47 kDa FOXP3 protein.
      BackgroundOne of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells. Several experiments using transgenic mice and retrovirus-mediated overexpression studies, have led to the identification of FOXP3, a transcription factor, as a specific molecular marker essential for the development and function of regulatory T cells. The primary evidence regarding the involvement of FOXP3 in the development of these cells was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of regulatory T cells and the role of FOXP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.
      HostMouse
      Immunogen speciesMurine
      Immunogenfull-length, recombinant, mouse FOXP3 containing a His•Tag® sequence
      EpitopeWithin the N-terminus
      Clone3G3
      IsotypeIgG1
      Specieshuman, mouse
      Positive controlLymph node cells
      FormLiquid
      FormulationIn PBS, 0.05% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤ 0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual sytems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659.