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PC168 Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb

This Anti-Estrogen Receptor β Rabbit pAb is validated for use in Frozen Sections, Gel Shift, WB, Paraffin Sections for the detection of Estrogen Receptor β.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ER β

PC168
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~60 kDa estrogen receptor β protein in overexpressing cell lines and breast carcinoma tissue. Does not cross-react with estrogen receptor α.
      Catalogue NumberPC168
      Brand Family Calbiochem®
      SynonymsAnti-ER β
      Application Data
      Detection of mouse estrogen receptor β by immunoblotting. Samples: I-11.15 cells (lane A), F9 cells (lane B) and NIH/3T3 cells (lane C) and whole cell lysates from mouse thymus cells (lane D) and spleen (lane E). Primary antibody: Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb (Cat. No. PC168) (1:1000). Detection: chemiluminescence.
      References
      ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinology 66, 63.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem. J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Positive controlBreast carcinoma tissue
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (5-10 µg/ml)
      Gel Shift (1-5 µl)
      Immunoblotting (1 µg/ml)
      Paraffin Sections (1:50)
      Application CommentsThis antibody will also specifically and efficiently supershift rat estrogen receptor β. Does not cross-react with estrogen receptor α. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (CSSTEDSKNKESSQNLQSQ) corresponding to amino acids 485-503 of rat estrogen receptor β
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      PC168 0