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CP63 Anti-EpCAM (Ab-1) Mouse mAb (1Dg.F3)

Anti-EpCAM (Ab-1) Mouse mAb (1Dg.F3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epithelial Cell Adhesion Molecule

CP63
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~40 kDa EpCAM protein.
      Catalogue NumberCP63
      Brand Family Calbiochem®
      SynonymsAnti-Epithelial Cell Adhesion Molecule
      Application Data
      Detection of human EpCAM by staining paraffin sections. Sample: Human breast carcinoma. Primary antibody: Anti-EpCAM (Ab-1) Mouse mAb (1Dg.F3) (Cat. No. CP63) (2.5 μg/ml). Detection: DAB
      References
      ReferencesPiyathilake, C. J., et al. 2000. Hum. Pathol. 31, 482.
      Ruck, P., et al. 2000. J. Pathol. 191, 102.
      Balzar, M., et al. 1999. J. Mol. Med. 77, 699.
      De Boer, C. J., et al. 1999. J Pathol. 188, 201.
      Poczatek, R. B., et al. 1999. J. Urol. 162, 1462.
      Zhong, X. Y., et al. 1999. Arch. Gynecol. Obstet. 263, 2.
      Litvinov, S. V., et al. 1994. J. Cell Biol. 125, 437.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50 % glycerol.
      Negative controlNormal mouse IgG
      Positive controlBreast carcinoma tissue
      PreservativeNone
      Applications
      Key Applications Not Western Blot
      Not Immunofluorescence
      Not Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (2.5 μg/ml, heat pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogennative, human EpCAM
      ImmunogenHuman
      Clone1Dg.F3
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP63 0

      Documentation

      Anti-EpCAM (Ab-1) Mouse mAb (1Dg.F3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EpCAM (Ab-1) Mouse mAb (1Dg.F3) Certificates of Analysis

      TitleLot Number
      CP63

      References

      Reference overview
      Piyathilake, C. J., et al. 2000. Hum. Pathol. 31, 482.
      Ruck, P., et al. 2000. J. Pathol. 191, 102.
      Balzar, M., et al. 1999. J. Mol. Med. 77, 699.
      De Boer, C. J., et al. 1999. J Pathol. 188, 201.
      Poczatek, R. B., et al. 1999. J. Urol. 162, 1462.
      Zhong, X. Y., et al. 1999. Arch. Gynecol. Obstet. 263, 2.
      Litvinov, S. V., et al. 1994. J. Cell Biol. 125, 437.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-Epithelial Cell Adhesion Molecule
      ApplicationImmunoblotting (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (2.5 μg/ml, heat pre-treatment required)
      Application Data
      Detection of human EpCAM by staining paraffin sections. Sample: Human breast carcinoma. Primary antibody: Anti-EpCAM (Ab-1) Mouse mAb (1Dg.F3) (Cat. No. CP63) (2.5 μg/ml). Detection: DAB
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~40 kDa EpCAM (Epithelial Cell Adhesion Molecule) protein.
      BackgroundEpCAM (epithelial cell adhesion molecule, GA733, CO17-1A, KSI-4, KSA, EGP, Trop-1) is an approximately 40 kDa integral membrane glycoprotein that is expressed on the baso-lateral cell surface in most human simple epithelia and in the vast majority of carcinomas. EpCAM functions to mediate Ca2+ independent homotypic cell-cell adhesion through interaction of its cytoplasmic tail with the actin cytoskeleton via α-actinin. EpCAM has been widely used to classify many different tumor types including but not restricted to LSCC, hepatic tumors, breast carcinoma, and prostatic tumors.
      HostMouse
      Immunogen speciesHuman
      Immunogennative, human EpCAM
      Clone1Dg.F3
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlBreast carcinoma tissue
      Negative controlNormal mouse IgG
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50 % glycerol.
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesPiyathilake, C. J., et al. 2000. Hum. Pathol. 31, 482.
      Ruck, P., et al. 2000. J. Pathol. 191, 102.
      Balzar, M., et al. 1999. J. Mol. Med. 77, 699.
      De Boer, C. J., et al. 1999. J Pathol. 188, 201.
      Poczatek, R. B., et al. 1999. J. Urol. 162, 1462.
      Zhong, X. Y., et al. 1999. Arch. Gynecol. Obstet. 263, 2.
      Litvinov, S. V., et al. 1994. J. Cell Biol. 125, 437.