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MABS1319 Anti-Cytochrome P450 26A1 Antibody, clone F27P6A1

Anti-Cytochrome P450 26A1, clone F27P6A1, Cat. No. MABS1319, is a highly specific mouse monoclonal antibody that targets Cytochrome P450 26A1 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CYP26A1, CYP26, P450RAI1 UniProt Number: O43174
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MABS1319
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1319
      DescriptionAnti-Cytochrome P450 26A1 Antibody, clone F27P6A1
      Alternate Names
      • Cytochrome P450 26A1
      • Cytochrome P450 retinoic acid-inactivating 1
      • Cytochrome P450RAI
      • hP450RAI
      • Retinoic acid 4-hydroxylase
      • Retinoic acid-metabolizing cytochrome
      Background InformationCytochrome P450 26A1 (UniProt O43174; also known as Cytochrome P450 retinoic acid-inactivating 1, Cytochrome P450RAI, hP450RAI, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome) is encoded by the CYP26A1 (also known as CYP26, P450RAI1) gene (Gene ID 1592) in human. CYP26A1 is a retinoic acid (RA) metabolizing enzyme that is mainly located in the endoplasmic reticulum membranes and is involved in vitamin A metabolism. It can generate several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. RA is a tight binding ligand for CYP26A1 with low nM binding affinity. Higher expression of CYP26A1 is observed in the adult liver, heart, pituitary gland, placenta, and various regions of the brain. CYP26A1 activity is important for the maintenance of pregnancy, especially during the process of blastocyst implantation. Elevated CYP26A1 expression and RA catabolic activity have been detected in breast epithelial adenocarcinoma cells in culture, in leukemic cells from patients with acute promyelocytic leukemia, and in cells derived from squamous cell carcinoma.

      Ref:
      Han BC et al., (2010). J. Cell Physiol. 223, 471-479.
      Osanai M., and Petkovich M (2005). Mol. Pharmacol. 67, 1808-1817.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytochrome P450 26A1, clone F27P6A1, Cat. No. MABS1319, is a highly specific mouse monoclonal antibody that targets Cytochrome P450 26A1 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected cytochrome P450 26A1 in human cerebellum, cerebral cortex, and large intestine tissue sections.

      Western Blotting Analysis: 4 µg/mL from a representative lot detected cytochrome P450 26A1 in 10 µg of human liver microsome lysate.

      Immunofluorescence Analysis: A representative lot immunostained dentate gyrus MAP2-positive neurons, but not GFAP-positive glia, nor CA1 hippocampal astrocytes and microglia by fluorescent immunohistochemistry staining of formalin-fixed, paraffin-embedded human hippocampus and cerebellum tissue sections (Stoney, P.N., et al. (2015). Brain Struct. Funct. In press).

      Immunohistochemistry Analysis: Representative lots detected differential cytochrome P450 26A1 immunoreactivity among formalin-fixed, paraffin-embedded tissue sections from normal colon, colon cancer, and lymph node metastasis (Brown, G.T., et al. (2014). PLoS One. 9(3):e90776; Kumarakulasingham, M., et al. (2005). Clin. Cancer Res. 11(10):3758-3765).

      Immunohistochemistry Analysis: A representative lot detected a significantly greater cytochrome P450 26A1 immunoreactivity among ormalin-fixed, paraffin-embedded ovarian cancer tissue sections when compared with normal ovary tissue sections (Downie, D., et al. (2005). Clin. Cancer Res.11(20):7369-7375).

      Western Blotting Analysis: A representative lot detected the ~54 kDa cytochrome P450 26A1 target band in human hippocampus tissue homogenate (Stoney, P.N., et al. (2015). Brain Struct. Funct. In press).

      Western Blotting Analysis: A representative lot detected the overexpression of cytochrome P450 26A1 exogenously transfected into human embryonic kidney cells (Brown, G.T., et al. (2014). PLoS One. 9(3):e90776).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to a sequence near the C-terminus of human cytochrome P450 26A1 (Kumarakulasingham, M., et al. (2005). Clin. Cancer Res. 11(10):3758-3765).
      CloneF27P6A1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityTarget band specificity was confirmed by Western blotting of lysate from cytochrome P450 26A1-overexpressing human embryonic kidney cells (Brown, G.T., et al. (2014). PLoS One. 9(3):e90776).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYP26A1
      • CYP26
      • P450RAI1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~53/48 kDa observed. 56.20/48.56 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human hippocampus tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected cytochrome P450 26A1 in 10 µg of human hippocampus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1319 04054839114458